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Human T-cell leukemia virus type I infection of monocytes and microglial cells in primary human cultures.

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  • 1. Retrovirus Research Center, Department of Veterans Affairs Medical Center, Baltimore, MD 21218.
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    • Hoffman PM 1
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Proceedings of the National Academy of Sciences of the United States of America, 01 Dec 1992, 89(24):11784-11788
https://doi.org/10.1073/pnas.89.24.11784 PMID: 1465399 PMCID: PMC50641

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Abstract 

The pathogenesis of progressive spastic paraparesis [HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP)], a serious consequence of human T-cell leukemia virus type I (HTLV-I) infection, is unclear. T and B lymphocytes can be naturally infected by HTLV-I, but the susceptibility to HTLV-I infection of other cell types that could contribute to the pathogenesis of HAM/TSP has not been determined. We found that a human monocyte cell line (THP-1), primary human peripheral blood monocytes, and isolated microglial cells but not astrocytes or oligodendroglial cells derived from adult human brain were infected by HTLV-I in vitro. Infection with HTLV-I enhanced the secretion of interleukin 6 in human microglial cell-enriched cultures but did not stimulate the release of interleukin 1 from monocytes or microglial cells. Tumor necrosis factor alpha production was stimulated by HTLV-I infection of monocytes and microglial cells and could be enhanced by suboptimal amounts of lipopolysaccharide. Since both tumor necrosis factor alpha and interleukin 6 have been implicated in inflammatory demyelination and gliosis, our findings suggest that human microglial cells and monocytes infected with and activated by HTLV-I could play a role in the pathogenesis of HAM/TSP.

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Proc Natl Acad Sci U S A. 1992 Dec 15; 89(24): 11784–11788.
PMCID: PMC50641
PMID: 1465399

Human T-cell leukemia virus type I infection of monocytes and microglial cells in primary human cultures.

P M Hoffman, S Dhib-Jalbut, J A Mikovits, D S Robbins, A L Wolf, G K Bergey, N C Lohrey, O S Weislow, and F W Ruscetti
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Abstract

The pathogenesis of progressive spastic paraparesis [HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP)], a serious consequence of human T-cell leukemia virus type I (HTLV-I) infection, is unclear. T and B lymphocytes can be naturally infected by HTLV-I, but the susceptibility to HTLV-I infection of other cell types that could contribute to the pathogenesis of HAM/TSP has not been determined. We found that a human monocyte cell line (THP-1), primary human peripheral blood monocytes, and isolated microglial cells but not astrocytes or oligodendroglial cells derived from adult human brain were infected by HTLV-I in vitro. Infection with HTLV-I enhanced the secretion of interleukin 6 in human microglial cell-enriched cultures but did not stimulate the release of interleukin 1 from monocytes or microglial cells. Tumor necrosis factor alpha production was stimulated by HTLV-I infection of monocytes and microglial cells and could be enhanced by suboptimal amounts of lipopolysaccharide. Since both tumor necrosis factor alpha and interleukin 6 have been implicated in inflammatory demyelination and gliosis, our findings suggest that human microglial cells and monocytes infected with and activated by HTLV-I could play a role in the pathogenesis of HAM/TSP.

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Selected References

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  • Akizuki S, Setoguchi M, Nakazato O, Yoshida S, Higuchi Y, Yamamoto S, Okajima T. An autopsy case of human T-lymphotropic virus type I-associated myelopathy. Hum Pathol. 1988 Aug;19(8):988–990. [Abstract] [Google Scholar]
  • Moore GR, Traugott U, Scheinberg LC, Raine CS. Tropical spastic paraparesis: a model of virus-induced, cytotoxic T-cell-mediated demyelination? Ann Neurol. 1989 Oct;26(4):523–530. [Abstract] [Google Scholar]
  • Wiley CA, Schrier RD, Nelson JA, Lampert PW, Oldstone MB. Cellular localization of human immunodeficiency virus infection within the brains of acquired immune deficiency syndrome patients. Proc Natl Acad Sci U S A. 1986 Sep;83(18):7089–7093. [Europe PMC free article] [Abstract] [Google Scholar]
  • Giulian D, Vaca K, Noonan CA. Secretion of neurotoxins by mononuclear phagocytes infected with HIV-1. Science. 1990 Dec 14;250(4987):1593–1596. [Abstract] [Google Scholar]
  • Pulliam L, Herndier BG, Tang NM, McGrath MS. Human immunodeficiency virus-infected macrophages produce soluble factors that cause histological and neurochemical alterations in cultured human brains. J Clin Invest. 1991 Feb;87(2):503–512. [Europe PMC free article] [Abstract] [Google Scholar]
  • Desrosiers RC, Hansen-Moosa A, Mori K, Bouvier DP, King NW, Daniel MD, Ringler DJ. Macrophage-tropic variants of SIV are associated with specific AIDS-related lesions but are not essential for the development of AIDS. Am J Pathol. 1991 Jul;139(1):29–35. [Europe PMC free article] [Abstract] [Google Scholar]
  • Mori K, Ringler DJ, Kodama T, Desrosiers RC. Complex determinants of macrophage tropism in env of simian immunodeficiency virus. J Virol. 1992 Apr;66(4):2067–2075. [Europe PMC free article] [Abstract] [Google Scholar]
  • Fan N, Gavalchin J, Paul B, Wells KH, Lane MJ, Poiesz BJ. Infection of peripheral blood mononuclear cells and cell lines by cell-free human T-cell lymphoma/leukemia virus type I. J Clin Microbiol. 1992 Apr;30(4):905–910. [Europe PMC free article] [Abstract] [Google Scholar]
  • Yong VW, Moumdjian R, Yong FP, Ruijs TC, Freedman MS, Cashman N, Antel JP. Gamma-interferon promotes proliferation of adult human astrocytes in vitro and reactive gliosis in the adult mouse brain in vivo. Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7016–7020. [Europe PMC free article] [Abstract] [Google Scholar]
  • Mikovits JA, Raziuddin, Gonda M, Ruta M, Lohrey NC, Kung HF, Ruscetti FW. Negative regulation of human immune deficiency virus replication in monocytes. Distinctions between restricted and latent expression in THP-1 cells. J Exp Med. 1990 May 1;171(5):1705–1720. [Europe PMC free article] [Abstract] [Google Scholar]
  • Hoffman PM, Cimino EF, Robbins DS. Effects of viral specific cytotoxic lymphocytes on the expression of murine leukemia virus induced neurologic disease. J Neuroimmunol. 1991 Aug;33(2):157–165. [Abstract] [Google Scholar]
  • Gonda MA. Molecular genetics and structure of the human immunodeficiency virus. J Electron Microsc Tech. 1988 Jan;8(1):17–40. [Abstract] [Google Scholar]
  • Byrne BC, Li JJ, Sninsky J, Poiesz BJ. Detection of HIV-1 RNA sequences by in vitro DNA amplification. Nucleic Acids Res. 1988 May 11;16(9):4165–4165. [Europe PMC free article] [Abstract] [Google Scholar]
  • Kwok S, Ehrlich G, Poiesz B, Kalish R, Sninsky JJ. Enzymatic amplification of HTLV-I viral sequences from peripheral blood mononuclear cells and infected tissues. Blood. 1988 Oct;72(4):1117–1123. [Abstract] [Google Scholar]
  • Kinoshita T, Shimoyama M, Tobinai K, Ito M, Ito S, Ikeda S, Tajima K, Shimotohno K, Sugimura T. Detection of mRNA for the tax1/rex1 gene of human T-cell leukemia virus type I in fresh peripheral blood mononuclear cells of adult T-cell leukemia patients and viral carriers by using the polymerase chain reaction. Proc Natl Acad Sci U S A. 1989 Jul;86(14):5620–5624. [Europe PMC free article] [Abstract] [Google Scholar]
  • Gendelman HE, Friedman RM, Joe S, Baca LM, Turpin JA, Dveksler G, Meltzer MS, Dieffenbach C. A selective defect of interferon alpha production in human immunodeficiency virus-infected monocytes. J Exp Med. 1990 Nov 1;172(5):1433–1442. [Europe PMC free article] [Abstract] [Google Scholar]
  • Jacobson S, Shida H, McFarlin DE, Fauci AS, Koenig S. Circulating CD8+ cytotoxic T lymphocytes specific for HTLV-I pX in patients with HTLV-I associated neurological disease. Nature. 1990 Nov 15;348(6298):245–248. [Abstract] [Google Scholar]
  • Ho DD, Rota TR, Hirsch MS. Infection of human endothelial cells by human T-lymphotropic virus type I. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7588–7590. [Europe PMC free article] [Abstract] [Google Scholar]
  • Saida T, Saida K, Funauchi M, Nishiguchi E, Nakajima M, Matsuda S, Ohta M, Ohta K, Nishitani H, Hatanaka M. HTLV-I myelitis: isolation of virus, genomic analysis, and infection in neural cell cultures. Ann N Y Acad Sci. 1988;540:636–638. [Abstract] [Google Scholar]
  • Yip MT, Chen IS. Modes of transformation by the human T-cell leukemia viruses. Mol Biol Med. 1990 Feb;7(1):33–44. [Abstract] [Google Scholar]
  • Tarella C, Ruscetti FW, Poiesz BJ, Woods A, Gallo RC. Factors that affect human hemopoiesis are produced by T-cell growth factor dependent and independent cultured T-cell leukemia-lymphoma cells. Blood. 1982 Jun;59(6):1330–1336. [Abstract] [Google Scholar]
  • Bhigjee AI, Wiley CA, Wachsman W, Amenomori T, Pirie D, Bill PL, Windsor I. HTLV-I-associated myelopathy: clinicopathologic correlation with localization of provirus to spinal cord. Neurology. 1991 Dec;41(12):1990–1992. [Abstract] [Google Scholar]
  • Turpin J, Mehta K, Blick M, Hester JP, Lopez-Berestein G. Effect of retinoids on the release and gene expression of tumor necrosis factor-alpha in human peripheral blood monocytes. J Leukoc Biol. 1990 Nov;48(5):444–450. [Abstract] [Google Scholar]
  • Yoshida M, Osame M, Kawai H, Toita M, Kuwasaki N, Nishida Y, Hiraki Y, Takahashi K, Nomura K, Sonoda S, et al. Increased replication of HTLV-I in HTLV-I-associated myelopathy. Ann Neurol. 1989 Sep;26(3):331–335. [Abstract] [Google Scholar]
  • Gessain A, Saal F, Gout O, Daniel MT, Flandrin G, de The G, Peries J, Sigaux F. High human T-cell lymphotropic virus type I proviral DNA load with polyclonal integration in peripheral blood mononuclear cells of French West Indian, Guianese, and African patients with tropical spastic paraparesis. Blood. 1990 Jan 15;75(2):428–433. [Abstract] [Google Scholar]
  • Gessain A, Caudie C, Gout O, Vernant JC, Maurs L, Giordano C, Malone G, Tournier-Lasserve E, Essex M, de-Thé G. Intrathecal synthesis of antibodies to human T lymphotropic virus type I and the presence of IgG oligoclonal bands in the cerebrospinal fluid of patients with endemic tropical spastic paraparesis. J Infect Dis. 1988 Jun;157(6):1226–1234. [Abstract] [Google Scholar]
  • Dhawan S, Streicher HZ, Wahl LM, Miller N, Louie AT, Goldfarb IS, Jackson WL, Casali P, Notkins AL. Model for studying virus attachment. II. Binding of biotinylated human T cell leukemia virus type I to human blood mononuclear cells potential targets for human T cell leukemia virus type I infection. J Immunol. 1991 Jul 1;147(1):102–108. [Abstract] [Google Scholar]
  • Poiesz BJ, Ruscetti FW, Mier JW, Woods AM, Gallo RC. T-cell lines established from human T-lymphocytic neoplasias by direct response to T-cell growth factor. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6815–6819. [Europe PMC free article] [Abstract] [Google Scholar]
  • Longo DL, Gelmann EP, Cossman J, Young RA, Gallo RC, O'Brien SJ, Matis LA. Isolation of HTLV-transformed B-lymphocyte clone from a patient with HTLV-associated adult T-cell leukaemia. Nature. 1984 Aug 9;310(5977):505–506. [Abstract] [Google Scholar]
  • Ehrlich GD, Davey FR, Kirshner JJ, Sninsky JJ, Kwok S, Slamon DJ, Kalish R, Poiesz BJ. A polyclonal CD4+ and CD8+ lymphocytosis in a patient doubly infected with HTLV-I and HIV-1: a clinical and molecular analysis. Am J Hematol. 1989 Mar;30(3):128–139. [Abstract] [Google Scholar]
  • Clapham P, Nagy K, Cheingsong-Popov R, Exley M, Weiss RA. Productive infection and cell-free transmission of human T-cell leukemia virus in a nonlymphoid cell line. Science. 1983 Dec 9;222(4628):1125–1127. [Abstract] [Google Scholar]
  • Chen IS, Slamon DJ, Rosenblatt JD, Shah NP, Quan SG, Wachsman W. The x gene is essential for HTLV replication. Science. 1985 Jul 5;229(4708):54–58. [Abstract] [Google Scholar]
  • Felber BK, Paskalis H, Kleinman-Ewing C, Wong-Staal F, Pavlakis GN. The pX protein of HTLV-I is a transcriptional activator of its long terminal repeats. Science. 1985 Aug 16;229(4714):675–679. [Abstract] [Google Scholar]
  • Siekevitz M, Feinberg MB, Holbrook N, Wong-Staal F, Greene WC. Activation of interleukin 2 and interleukin 2 receptor (Tac) promoter expression by the trans-activator (tat) gene product of human T-cell leukemia virus, type I. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5389–5393. [Europe PMC free article] [Abstract] [Google Scholar]
  • Cross SL, Feinberg MB, Wolf JB, Holbrook NJ, Wong-Staal F, Leonard WJ. Regulation of the human interleukin-2 receptor alpha chain promoter: activation of a nonfunctional promoter by the transactivator gene of HTLV-I. Cell. 1987 Apr 10;49(1):47–56. [Abstract] [Google Scholar]
  • Fujii M, Sassone-Corsi P, Verma IM. c-fos promoter trans-activation by the tax1 protein of human T-cell leukemia virus type I. Proc Natl Acad Sci U S A. 1988 Nov;85(22):8526–8530. [Europe PMC free article] [Abstract] [Google Scholar]
  • Miyatake S, Seiki M, Yoshida M, Arai K. T-cell activation signals and human T-cell leukemia virus type I-encoded p40x protein activate the mouse granulocyte-macrophage colony-stimulating factor gene through a common DNA element. Mol Cell Biol. 1988 Dec;8(12):5581–5587. [Europe PMC free article] [Abstract] [Google Scholar]
  • Watkins BA, Dorn HH, Kelly WB, Armstrong RC, Potts BJ, Michaels F, Kufta CV, Dubois-Dalcq M. Specific tropism of HIV-1 for microglial cells in primary human brain cultures. Science. 1990 Aug 3;249(4968):549–553. [Abstract] [Google Scholar]
  • Merrill JE, Koyanagi Y, Zack J, Thomas L, Martin F, Chen IS. Induction of interleukin-1 and tumor necrosis factor alpha in brain cultures by human immunodeficiency virus type 1. J Virol. 1992 Apr;66(4):2217–2225. [Europe PMC free article] [Abstract] [Google Scholar]
  • Kurt-Jones EA, Beller DI, Mizel SB, Unanue ER. Identification of a membrane-associated interleukin 1 in macrophages. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1204–1208. [Europe PMC free article] [Abstract] [Google Scholar]
  • Kriegler M, Perez C, DeFay K, Albert I, Lu SD. A novel form of TNF/cachectin is a cell surface cytotoxic transmembrane protein: ramifications for the complex physiology of TNF. Cell. 1988 Apr 8;53(1):45–53. [Abstract] [Google Scholar]
  • Dinarello CA. Interleukin-1 and interleukin-1 antagonism. Blood. 1991 Apr 15;77(8):1627–1652. [Abstract] [Google Scholar]
  • Lindholm PF, Marriott SJ, Gitlin SD, Bohan CA, Brady JN. Induction of nuclear NF-kappa B DNA binding activity after exposure of lymphoid cells to soluble tax1 protein. New Biol. 1990 Nov;2(11):1034–1043. [Abstract] [Google Scholar]
  • Robbins DS, Shirazi Y, Drysdale BE, Lieberman A, Shin HS, Shin ML. Production of cytotoxic factor for oligodendrocytes by stimulated astrocytes. J Immunol. 1987 Oct 15;139(8):2593–2597. [Abstract] [Google Scholar]
  • Selmaj K, Raine CS, Farooq M, Norton WT, Brosnan CF. Cytokine cytotoxicity against oligodendrocytes. Apoptosis induced by lymphotoxin. J Immunol. 1991 Sep 1;147(5):1522–1529. [Abstract] [Google Scholar]
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