Structural basis for broad neutralization of HIV-1 through the molecular recognition of 10E8 helical epitope at the membrane interface

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Published online 2016 Dec 1. doi: 10.1038/srep38177

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Table 2

Thermodynamic parameters of binding of Fab 10E8 CDRH3 muteins to the peptide 10E8ep.

Antibody	K_D(nM)	ΔG° (kcal mol⁻¹)	ΔH° (kcal mol⁻¹)	−TΔS° (kcal mol⁻¹)^a	n^b
WT	9.6±1.0	−10.9±0.1	−9.6±0.1	−1.3±0.2	1.0±0.1
Pro100f_HCAla	161±22	−9.2±0.1	−11.1±0.2	1.9±0.3	0.8±0.1
Trp100b_HCTyr	52±12	−9.9±0.1	−8.9±0.1	−1.0±0.2	1.1±0.1
Trp100b_HCAla	361±82	−8.8±0.1	−10.0±0.5	1.2±0.6	1.0±0.1
Trp100b_HCAsp	375±66	−8.7±0.1	−6.5±0.3	−2.2±0.4	1.0±0.1

^aTemperature was 298K.

^bn refers to the molar ratio peptide/protein.