FIG 2

DENV4 neutralizing hMAb D4-126 or D4-131 preferentially binds to epitopes displayed on intact virions. (A) U937+DC-SIGN flow cytometry-based neutralization assays were conducted to determine the neutralization of DENV1 to DENV4 by D4-126 or D4-131. Results are from 2 technical replicates in an experiment. Each point represents the mean neutralization value from the two replicates, and the error bars depict the standard deviations. Neutralization experiments were repeated at least twice. (B) Binding to DENV4 whole virions, recombinant E protein ectodomain (rE), and recombinant E protein domain III (rEDIII) by D4-126 or D4-131 was tested in an ELISA. Results are from 2 technical replicates in one experiment. Each point represents the mean from the two replicates, and the error bars depict the standard deviations OD, optical density.