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Published online 2017 Jun 28. doi: 10.1186/s12885-017-3442-y
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Fig. 5

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Knockdown of MCL-1 sensitizes ESCC cells to cisplatin-mediated apoptosis. a, b Stable KYSE150 (a) and KYSE510 (b) cells with MCL-1 knockdown were starved in 0.1% FBS/RPMI 1640 medium overnight and then cultured without (DMSO) or with different concentrations of cisplatin in 10% FBS/RPMI 1640 medium for 48 h. After treatment, attached and floating cells were harvested. Cleavage of caspase-3 and PARP were analyzed by Western blotting. β-actin was used as a loading control. Arrow head: 17KD or19 KD of cleaved caspase-3

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