Figure S5

Evaluation of apoptosis of MDR-1 siRNA-Dox-SiO₂ containing different Dox concentrations determined by flow cytometry using annexin V method in MCF-7/MDR cells. (A) control, without any treatment; (B) Free dox, dox concentration of 2000 ng/mL; (C) NS siRNA-SiO₂; (D) NS siRNA-Dox-SiO₂, Dox concentration of 2000 ng/mL; (E) MDR-1 siRNA-Dox-SiO₂-1, Dox concentration at 500 ng/mL; (F) MDR-1 siRNA-Dox-SiO₂-2, dox concentration at 1000 ng/mL; (G) MDR-1 siRNA-Dox-SiO₂-3, Dox concentration at 2000 ng/mL; (H) Quantitation of apoptotic rate of MCF-7/MDR cells after treatment with different groups. All of groups contained the same siRNA value. M5% represents apoptotic cell population%. *p<0.01; **p<0.001; ***p<0.0001, n=3.

Abbreviations: NC siRNA, negative small interfering RNA; Dox, doxorubicin; SiO₂, silica.