Figure 2

Indirect co-culture with ASCs induces cell cycle arrest in Huh7 cells without apoptosis. Huh7 cells were co-cultured with ASCs at doses of 40,000 cells/cm2 for 2 days. (A) Micrographs of light microscopy of Huh7 cells co-cultured with ASCs (left image) or not (right image) at day 2. 40 x magnification. (B) MTT assay showing cell viability of Huh7 cells co-cultured with ASCs or not at day 1 and day 2. (C) Western blot analysis indicating relative amount of PCNA, PARP, p53, p21, STAT1, p-STAT1 and GAPDH in lysates of Huh7 cells co-cultured with ASCs at day 1 and day 2. (D) FACS analyses of DNA content in Huh7 cells co-cultured with ASCs at day 1 and day 2. (E) Cell cycle distribution of Huh7 cells co-cultured with ASCs at day 1 and day 2. Error bars represent the mean ± SD from triplicate analyses. Data were obtained from one of three independent experiments. *P < 0.05. ns, no statistical significance.