RGD Reference Report - Induction of antigen specific cellular immunity by vaccination with peptides from MN/CA IX in renal cell carcinoma. - Rat Genome Database

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Induction of antigen specific cellular immunity by vaccination with peptides from MN/CA IX in renal cell carcinoma.

Authors: Shimizu, K  Uemura, H  Yoshikawa, M  Yoshida, K  Hirao, Y  Iwashima, K  Saga, S  Yoshikawa, K 
Citation: Shimizu K, etal., Oncol Rep. 2003 Sep-Oct;10(5):1307-11.
RGD ID: 2293199
Pubmed: PMID:12883698   (View Abstract at PubMed)

We investigated the induction of the specific immunity for renal cell carcinomas (RCC) using MN/CA IX, a tumor-associated antigen frequently expressed in RCC. We have generated 9-mer peptide derived from MN/CA IX and examined the antigenicity as a vaccine to induce specific immunity for RCC. To use mouse syngeneic system, we transfected human MN/CA9 cDNA into RenCa and BALB-3T3 cells originally from BALB/c mouse, and established MN/CA IX expressing mouse cell lines, i.e., MN-RenCa and MN-3T3. The immunization of BALB/c mouse with MN-RenCa cells resulted in the induction of cytotoxic T lymphocytes (CTL) against MN/CA IX expressing cells and the CTL clone was established from bulked CTL. This CTL clone specifically lyzed MN-3T3 cells, but not parental cells. To identify the targeted epitope binding to H-2Kd antigen, three 9-mer peptides (A, B, C-peptide) of human MN/CA IX compatible with the H-2Kd as well as HLA-A24 binding motif was synthesized. The cloned CTL targeted the B-peptide pulsed BALB-3T3 cells as well as MN-3T3 cells. Furthermore, spleen cells from BALB/c mouse immunized with B-peptide reacted against MN-RenCa cells. These results suggest that the peptides derived from MN/CA IX containing HLA-A24 binding motif may be useful as a potent tumor vaccine for the treatment of human RCC, and in mouse models.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CA9Humanrenal cell carcinoma  IDA  RGD 
Car9Ratrenal cell carcinoma  ISOCA9 (Homo sapiens) RGD 
Car9Mouserenal cell carcinoma  ISOCA9 (Homo sapiens) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Car9  (carbonic anhydrase 9)

Genes (Mus musculus)
Car9  (carbonic anhydrase 9)

Genes (Homo sapiens)
CA9  (carbonic anhydrase 9)


Additional Information