RGD Reference Report - Hypomethylation of the MN/CA9 promoter and upregulated MN/CA9 expression in human renal cell carcinoma. - Rat Genome Database

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Hypomethylation of the MN/CA9 promoter and upregulated MN/CA9 expression in human renal cell carcinoma.

Authors: Cho, M  Uemura, H  Kim, SC  Kawada, Y  Yoshida, K  Hirao, Y  Konishi, N  Saga, S  Yoshikawa, K 
Citation: Cho M, etal., Br J Cancer. 2001 Aug 17;85(4):563-7.
RGD ID: 2293201
Pubmed: PMID:11506497   (View Abstract at PubMed)
PMCID: PMC2364093   (View Article at PubMed Central)
DOI: DOI:10.1054/bjoc.2001.1951   (Journal Full-text)

MN/CA9 is a cancer-related gene, frequently activated in human renal cell carcinomas (RCCs). To reveal the activation mechanism, we investigated the relationship between methylation status of the MN/CA9 promoter region and gene expression using 13 human RCCs, and examined the effect of in vitro CpG methylation on the MN/CA9 promoter activity using a human RCC cell line (SK-RC-44), expressing MN/CA9. MN/CA9 expression was evaluated by RT-PCR and observed in 10 of 13 RCCs (77%). A total of 9 out of 10 MN/CA9 -positive RCCs (90%) contained clear cell components. Methylation status of 6 CpGs in the MN/CA9 promoter region was decided by using the bisulfite genomic sequencing protocol. Out of 13 RCCs 9 (69%) showed partial hypomethylation of the CpG at -74 bp, while the other 4 RCCs and 3 normal kidney tissue samples showed complete methylation. Hypomethylation of the CpG at -74 bp was strongly correlated with MN/CA9 expression. Luciferase assay revealed that the MN/CA9 promoter activity was strongly suppressed by methylation of the CpG at -74 bp. These findings suggest that hypomethylation of the CpG at -74 bp in the MN/CA9 promoter region might play an important role in this gene activation of human RCC.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CA9Humanrenal cell carcinoma  IDA DNA more ...RGD 
Car9Ratrenal cell carcinoma  ISOCA9 (Homo sapiens)DNA more ...RGD 
Car9Mouserenal cell carcinoma  ISOCA9 (Homo sapiens)DNA more ...RGD 

Objects Annotated

Genes (Rattus norvegicus)
Car9  (carbonic anhydrase 9)

Genes (Mus musculus)
Car9  (carbonic anhydrase 9)

Genes (Homo sapiens)
CA9  (carbonic anhydrase 9)


Additional Information