KEGG ENZYME: 3.4.19.16

ENZYME: 3.4.19.16

Entry

EC 3.4.19.16                Enzyme

Name

glucosinolate gamma-glutamyl hydrolase;
GGP1 (gene name);
GGP3 (gene name)

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Omega peptidases

Reaction(IUBMB)

(1) an (E)-1-(glutathion-S-yl)-N-hydroxy-omega-(methylsulfanyl)alkan-1-imine + H2O = an (E)-1-(L-cysteinylglycin-S-yl)-N-hydroxy-omega-(methylsulfanyl)alkan-1-imine + L-glutamate [RN:R11792];
(2) (E)-1-(glutathion-S-yl)-N-hydroxy-2-(1H-indol-3-yl)ethan-1-imine + H2O = (E)-1-(L-cysteinylglycin-S-yl)-N-hydroxy-2-(1H-indol-3-yl)ethan-1-imine + L-glutamate [RN:R11793];
(3) (glutathion-S-yl)(1H-indol-3-yl)acetonitrile + H2O = (L-cysteinylglycin-S-yl)(1H-indol-3-yl)acetonitrile + L-glutamate [RN:R11794];
(4) (Z)-1-(glutathion-S-yl)-N-hydroxy-2-phenylethan-1-imine + H2O = (Z)-1-(L-cysteinyglycin-S-yl)-N-hydroxy-2-phenylethan-1-imine + L-glutamate [RN:R11795]

Reaction(KEGG)

R11792 R11793 R11794 R11795

Substrate

(E)-1-(glutathion-S-yl)-N-hydroxy-omega-(methylsulfanyl)alkan-1-imine;
H2O [CPD:C00001];
(E)-1-(glutathion-S-yl)-N-hydroxy-2-(1H-indol-3-yl)ethan-1-imine;
(glutathion-S-yl)(1H-indol-3-yl)acetonitrile;
(Z)-1-(glutathion-S-yl)-N-hydroxy-2-phenylethan-1-imine

Product

(E)-1-(L-cysteinylglycin-S-yl)-N-hydroxy-omega-(methylsulfanyl)alkan-1-imine;
L-glutamate [CPD:C00025];
(E)-1-(L-cysteinylglycin-S-yl)-N-hydroxy-2-(1H-indol-3-yl)ethan-1-imine;
(L-cysteinylglycin-S-yl)(1H-indol-3-yl)acetonitrile;
(Z)-1-(L-cysteinyglycin-S-yl)-N-hydroxy-2-phenylethan-1-imine

Comment

This enzyme, characterized from the plant Arabidopsis thaliana, participates in the biosynthesis of the plant defense compounds glucosinolates and camalexin. It is the only known plant enzyme capable of hydrolysing the gamma-glutamyl residue of glutathione in the cytosol.

History

EC 3.4.19.16 created 2017

Orthology

K22314

glucosinolate gamma-glutamyl hydrolase

Genes

NVE:

5515324

EPA:	110235103 110236718 110255075

ATEN:

116299149

ADF:

107348401

AMIL:

114964847

PDAM:

113677131

SPIS:

111327171

DGT:

114526438

XEN:

124440256

HMG:	101240492 105844229

HSY:	130612435 130622308 130623845 130642202

RES:	135694783 135694829 135695003

ATH:	AT2G23960 AT2G23970 AT4G30530(GGP1) AT4G30540 AT4G30550(GGP3)

ALY:	9303414 9305443 9305444 9316603

CRB:	17878381 17878489 17880012 17880488 17890112

CSAT:

104702915 104713836 104717139 104717141 104717142 104721790 104721793 104721794 104730255 104730257 104730258 104752025 104774366

EUS:	EUTSA_v10000299mg EUTSA_v10000640mg EUTSA_v10026067mg EUTSA_v10026083mg EUTSA_v10027000mg

BRP:	103834667 103834668 103842392 103842394 103852592 103861974

BNA:	106365159 106368635 106392894 106400381 106408952 106414873 106416083 106424103 106424211 125583484 125583485 125590147 125609478

BOE:	106306103 106306562 106310578 106312467 106329223 106329266

RSZ:	108809618 108813611 108816757 108831369 108833915 108833916 108851551 130501945 130504970 130505449 130505658 130508167

THJ:	104803534 104803536 104803539 104803921 104807184 104819995

CPAP:

110819018

CIT:	102624397 102629214

CIC:	CICLE_v10009284mg CICLE_v10029932mg

PVY:	116121936 116121937 116142064 116143811

MINC:

123194511 123195200 123199885 123201146 123201149 123213117

TCC:	18589037 18608189

GRA:	105779911 105782861 105791012 105795123

GHI:	107886999 107894173 107898771 107907726 107920347 107945175 121204599 121225719

GAB:	108459694 108461703 108474421 108482468

HSYR:

120115880 120116496 120120593 120134194 120145170 120149827 120171611 120177480 120177953 120210568 120212013 120214909

DZI:	111282511 111282881 111285067 111295545 111304248 111309066

EGR:	104419874 104419875 104436013 104436014

GMX:	100777176 100794668 100808286 100813163

GSJ:	114375643 114393199 114419424 114422977

PVU:	137805904 137811898 137832743

VRA:	106767438 106767996 106776292

VAR:	108320294 108341076 108341427

VUN:	114175230 114179829 114187141 114188220

VUM:	124823192 124831135 124836648

CCAJ:

109803173 109804642

APRC:

113847132 113852959 113859095

MTR:	11407738 11431148 11433871

TPRA:

123910556 123911308 123912780 123922353

CAM:	101488889 101497879 101509568 101509890

PSAT:

127084034 127106184

VVO:	131604850 131624892 131631347 131633881

LJA:	130715592 130716216 130726296

ADU:	107459585 107468739 107480452

AIP:	107624114 107631057 107641548

AHF:	112699490 112714793 112723474 112733382 112784254 112791316

LANG:

109326195 109329052 109332015 109334535 109349298

PCIN:

129285814 129305370 129306780 129307425 129307877

QSA:	O6P43_012441 O6P43_022235 O6P43_031678

FVE:	101306191 101310360

RCN:	112171161 112200289

PPER:

18767070 18792319

PMUM:

103323428 103335981

PAVI:

110759102 110764109

PDUL:

117612402 117615658

MDM:	103412048 103428242 114822053 114824755

MSYL:

126585490 126597077 126607360 126620703

PXB:	103929232 103935733 103942059 103950509 103956111 103957818

ZJU:	107425741 107426808

MNT:	21391927 21397902

CSAV:

115694907 115719792 115724737

CSV:	101205790 101206066 101215620

CMO:	103494858 103495371

BHJ:	120077669 120091938

MCHA:

111007348 111008451 111017631

CMAX:

111470593 111482610 111487691

CMOS:

111430531 111448037 111455260

CPEP:

111787978 111810368 111811902

RCU:	8265758 8279073

MEAN:

126660031 126664716

JCU:	105633567 105633617 105641450

HBR:	110635404 110638732 110647629 110648296

MESC:

110616952 110618732 110623930

POP:	7454830 7493370

PEU:	105110012 105121222

PALZ:

118027613 118034263

PNZ:	133674185 133679321

JRE:	108983914 108989512 108993096 108995673

CILL:

122297206 122299526 122309085 122312454

CAVE:

132175378 132178807 132185614

QSU:	112009412 112037074 136066620

QLO:	115958950 115967436

TWL:	119982074 119984548 119993286

VVI:	100259085 100267393

VRI:	117910103 117911279 117925440

SLY:	101254399 101267066

SPEN:

107009125 107025647

SOT:	102585222 102585501

SSTN:

125850894 125877659

SDUL:

129885711 129893111

CANN:

107840110 107854225

LBB:	132598855 132602901

NTA:	107765602 107772668 107794450 107809941 107830013

NSY:	104212375 104218893 104249304

NTO:	104110542 104111543

NAU:	109221059 109229136

INI:	109161240 109165058 109166351

ITR:	116026325 116028479 116033482

SIND:

105155619 105169292 105172639

OEU:	111374295 111374296 111380734 111386638 111389020 111399606 111410202 111410677

EGT:	105949599 105956101 105958351

SSPL:

121755386 121758298 121803355 121806373

SMIL:

131005034 131005171

SHIS:

125193226 125223422

APAN:

127251472 127253509 127262449 127264719

HAN:	110917519 110929502 110940260

ECAD:

122593758 122594670 122598474 122600190

LSV:	111899051 111906921 111913274

CCAV:

112500408 112507411 112517466

DCR:	108211617 108221955 108225446

CSIN:

114282624 114288256 114296361

RVL:	131308059 131320358 131325453

AEW:	130752680 130754956 130777397 130778972

BVG:	104901749 104904768

SOE:	110801426 110805905

CQI:	110685084 110715792 110721737 110740064

ATRI:

130797458 130806638

MOF:	131157351 131157609 131157721 131157965 131164769

NNU:	104589638 104589829 104589830 104589831 104590921 104595528 104605395 104605396 104605398 104605399 104605400 104605401 104605402 104605403 104611953

MING:

122071598 122071696 122073589 122074705 122075703 122080199 122080785 122080838 122080883 122083572 122088717 122089695

TSS:	122644724 122657645 122659748 122660540 122660541 122661131 122662310 122662381 122662395 122663021

PSOM:

113349692 113357469

OSA:	4328499 4333748 4347102

OBR:	102702165 102712010 102714995

OGL:	127762879 127768963 127784692

BDI:	100824869 100831672 100832775

ATS:	109745571 109750656 109750657 109750658 109781866

TDC:	119287046 119294673 119299569 119299571 119309613 119309614 119309615 119316287 119325600

TAES:

123087255 123090702 123095714 123101359 123101360 123114806 123114807 123114808 123120582 123120583 123120584 123120585 123120586 123130467 123133956 123141333

TUA:	125507075 125507076 125507077 125513826

LPER:

127293413 127298152 127308077

LRD:	124651489 124651522 124687988 124689810 124697093 124700490

SBI:	8055997 8067265 8077619 8079398

ZMA:	100284196 100284640 103647442 103654868

MFLO:

136462821 136462822 136462823 136462867 136471222 136471274 136530829 136538420

SITA:

101760989 101761784 101762195 101775351 101784304

SVS:	117838578 117841738 117844244 117845678 117864371

PVIR:

120648314 120653915 120654837 120658852 120672002 120691003 120695695

PHAI:

112872898 112877917 112881086 112902340

PDA:	103707253 103709804 103710194 120107775

EGU:	105034878 105039558 105049884 105053909

MUS:	103999020 135610196 135611849 135615604 135619551

ZOF:	121969220 121990468 121994285 122008465 122017323 122027347

DCT:	110102497 110103081 110113354

PEQ:	110020638 110023481

AOF:	109832231 109833835 109842305

MSIN:

131218713 131229409 131256889

ACAK:

137936560 137942050 137947012 137950206 137950882 137951778 137953320

NCOL:

116260043 116260534 116261033

ATR:	110007822 18435338 18440851 18440856 18440857

SMO:	SELMODRAFT_110438 SELMODRAFT_128284

PPP:

112285930

CSL:	COCSUDRAFT_53611 COCSUDRAFT_61836

CVR:	CHLNCDRAFT_141352

PLJ:	28893120(PLICBS_009913)

CDET:

87936749(CDEST_00246)

PFY:	PFICI_02363 PFICI_15047

PSCO:

LY89DRAFT_719534

GLZ:	GLAREA_00276

AOR:	AO090011000566

PCS:	N7525_000317 N7525_007826

NPA:	UCRNP2_3721

WSE:	WALSEDRAFT_13494

WIC:	J056_004110

PFP:	PFL1_06599

ACAN:

ACA1_264000

TET:	TTHERM_00242040

PTM:	GSPATT00001723001 GSPATT00009055001 GSPATT00025206001

DSA:	Desal_3256

» show all

Reference

1 [PMID:21712415]

Authors

Geu-Flores F, Moldrup ME, Bottcher C, Olsen CE, Scheel D, Halkier BA

Title

Cytosolic gamma-glutamyl peptidases process glutathione conjugates in the biosynthesis of glucosinolates and camalexin in Arabidopsis.

Journal

Plant Cell 23:2456-69 (2011)
DOI:10.1105/tpc.111.083998

Sequence

[ath:AT4G30530 AT4G30550]

Other DBs

ExplorEnz - The Enzyme Database:

3.4.19.16

IUBMB Enzyme Nomenclature:

3.4.19.16

ExPASy - ENZYME nomenclature database:

3.4.19.16

BRENDA, the Enzyme Database:

3.4.19.16

LinkDB

DBGET integrated database retrieval system