KEGG ENZYME: 6.3.4.23

ENZYME: 6.3.4.23

Entry

EC 6.3.4.23                 Enzyme

Name

formate---phosphoribosylaminoimidazolecarboxamide ligase;
5-formaminoimidazole-4-carboxamide ribonucleotide synthetase;
5-formaminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate synthetase;
purP (gene name)

Class

Ligases;
Forming carbon-nitrogen bonds;
Other carbon-nitrogen ligases

Sysname

formate:5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide ligase (ADP-forming)

Reaction(IUBMB)

ATP + formate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = ADP + phosphate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide [RN:R06975]

Reaction(KEGG)

R06975

Substrate

ATP [CPD:C00002];
formate [CPD:C00058];
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide [CPD:C04677]

Product

ADP [CPD:C00008];
phosphate [CPD:C00009];
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide [CPD:C04734]

Comment

This archaeal enzyme, characterized from the methanogen Methanocaldococcus jannaschii, catalyses a step in the synthesis of purine nucleotides. It differs from the orthologous bacterial/eukaryotic enzymes, which utilize 10-formyltetrahydrofolate rather than formate and ATP. cf. EC 2.1.2.3, phosphoribosylaminoimidazolecarboxamide formyltransferase.

History

EC 6.3.4.23 created 2013

Pathway

ec00230

Purine metabolism

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K06863

5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase

Genes

VAB:	WPS_12340 WPS_12360

PRF:	PeribacterA2_0725 PeribacterA2_0726

MJA:

MJ_0136

MFE:	Mefer_0308

MVU:	Metvu_0168

MFS:	MFS40622_1004

MIF:	Metin_0767

MJH:	JH146_0737

MESG:

MLAUSG7_1026(purP)

MESA:

MLASG1_0751(purP)

MIG:	Metig_0553

MMP:

MMP1373

MMQ:	MmarC5_0205

MMX:	MmarC6_1300

MMZ:	MmarC7_0618

MMD:	GYY_07695(purP)

MMAK:

MMKA1_15970(purP)

MMAO:

MMOS7_14780(purP)

MMAD:

MMJJ_14660

MAE:

Maeo_1327

MVN:	Mevan_0683

MVO:

Mvol_0265

MOK:	Metok_0595

METF:

CFE53_06120(purP)

MTH:

MTH_1201

MMG:	MTBMA_c15790(purP)

MWO:	MWSIV6_1594(purP)

MTEE:

MTTB_02260(purP)

METC:

MTCT_1094

METE:

tca_01158

METZ:

METMT2_1203

METK:

FVF72_05615

MTHM:

FZP57_07640

MST:

Msp_0240

METB:

AW729_05985(purP)

MEIS:

PXD04_04980

MRU:	mru_0595(purP)

MSI:

Msm_0506

MEB:	Abm4_1385(purP)

MMIL:

sm9_0511(purP)

MEYE:

TL18_02305(purP)

MOL:

YLM1_0256

MARO:

MarbSA_08230(purP)

METV:

K4897_03040

MEL:	Metbo_0324

MEW:	MSWAN_2096

METH:

MBMB1_0083(purP)

MFC:	BRM9_0147(purP)

MFI:	DSM1535_1470(purP)

MCUB:

MCBB_2033(purP)

MSUB:

BK009_10205(purP)

METN:

BK008_05830(purP)

METT:

CIT01_03875(purP)

METO:

CIT02_06600(purP)

MEME:

HYG87_05155

MFEG:

GCM10025860_03410(purP)

MEER:

NKF70_05370

MFV:

Mfer_0393

MKA:

MK0588

AFU:	AF_0113 AF_0256

AFG:	AFULGI_00001100 AFULGI_00002440

APO:	Arcpr_1226 Arcpr_1231

AVE:	Arcve_0590 Arcve_1105

AST:	Asulf_00339 Asulf_00414

FPL:	Ferp_0934 Ferp_1822

GAC:	GACE_1955 GACE_2044

GAH:	GAH_00649 GAH_00717

PFU:	PF0421 PF1517

PFI:	PFC_01190 PFC_06820(purP)

PHO:	PH0324(PH0324) PH1348(PH1348)

PAB:	PAB0547 PAB1272

PYN:	PNA2_0941 PNA2_1924

PYA:	PYCH_06270 PYCH_10750 PYCH_16070

PYS:	Py04_0506 Py04_1238

PYC:	TQ32_03685 TQ32_10365

TKO:	TK0196 TK0203 TK0431

TGA:	TGAM_0751(purP) TGAM_0757 TGAM_1267

TSI:	TSIB_0049 TSIB_0059

TBA:	TERMP_02032 TERMP_02040

THE:	GQS_02000(purP) GQS_05740(purP) GQS_05765

THA:	TAM4_1003 TAM4_813

THM:	CL1_0040 CL1_0780 CL1_0784

TLT:	OCC_05274 OCC_06571 OCC_06656

TNU:	BD01_0510 BD01_1742 BD01_1747

TEU:	TEU_00655 TEU_00705 TEU_09520

TGY:	X802_06580 X802_06610 X802_10035

THV:	ADU37_CDS22240 ADU37_CDS22310 ADU37_CDS22690

TCH:	CHITON_1151 CHITON_1566

TPEP:

A0127_02245 A0127_02990 A0127_03015

TPIE:

A7C91_05460 A7C91_05480 A7C91_09085

TCE:	A3L02_01430 A3L02_01515 A3L02_01970

TBS:	A3L01_00110 A3L01_00130

THH:	CDI07_00150 CDI07_00175

TSL:	A3L11_03840 A3L11_03860

TTD:	A3L14_01400 A3L14_01430 A3L14_05520

TPRF:

A3L09_06380 A3L09_08495 A3L09_08525

TRL:	A3L10_09820 A3L10_09840

TPAF:

A3L08_03260 A3L08_03280 A3L08_06910

THY:	A3L12_03075 A3L12_03145 A3L12_03165

TIC:	FH039_02150 FH039_02230 FH039_06480

TCQ:	TIRI35C_0238(purP) TIRI35C_0244(purP) TIRI35C_0997

THEM:

FPV09_02900 FPV09_07110 FPV09_07135

THEI:

K1720_03315 K1720_03365

PPAC:

PAP_00555 PAP_00655

MBA:	Mbar_A0288 Mbar_A1334

MBY:	MSBRM_0231 MSBRM_2665

MBW:	MSBRW_0708 MSBRW_3391

MBAR:

MSBR2_2150 MSBR2_2623

MBAK:

MSBR3_2218 MSBR3_2802

MAC:	MA_0206 MA_3689

MMA:	MM_0593 MM_1493 MM_2913

MMAZ:

MmTuc01_0600 MmTuc01_1548 MmTuc01_2988

MMJ:	MSMAS_0716 MSMAS_1802 MSMAS_3175

MMAC:

MSMAC_0717 MSMAC_1314 MSMAC_3195

MVC:	MSVAZ_0238 MSVAZ_0647

MEK:	MSKOL_0231 MSKOL_0625

MLS:	MSLAZ_0127 MSLAZ_2268

METM:

MSMTP_0909 MSMTP_3081

MEF:	MSWH1_0142 MSWH1_2406

MEQ:	MSWHS_0140 MSWHS_2641

MSJ:	MSSAC_0179 MSSAC_3519

MSZ:	MSSIH_0165 MSSIH_3038

MSW:	MSSIT_0169 MSSIT_3096

MTHR:

MSTHT_1985 MSTHT_2423

MTHE:

MSTHC_0859 MSTHC_1299

MHOR:

MSHOH_0831 MSHOH_2312 MSHOH_3913

MFZ:	AOB57_003310 AOB57_009245

MBU:	Mbur_1029(purP) Mbur_1626(purP)

MMET:

MCMEM_1511 MCMEM_2123

MELO:

J7W08_06365 J7W08_09580

MMH:	Mmah_0026 Mmah_1404

MHAZ:

BHR79_03335 BHR79_06655

MPOT:

BKM01_02900 BKM01_10575

MEV:	Metev_0086 Metev_0565

MZH:	Mzhil_0081 Mzhil_0477

MPY:	Mpsy_0967(purP) Mpsy_1916

MZI:	HWN40_03700 HWN40_12625

MMAV:

RE476_06515 RE476_10895

MSEB:

RE474_01995 RE474_07470

MHZ:	Metho_0136 Metho_0550

MEHF:

MmiHf6_07840 MmiHf6_15440

MEES:

MmiEs2_00040 MmiEs2_03160

MTP:	Mthe_0184 Mthe_1099

MCJ:	MCON_1268 MCON_3207

MHI:	Mhar_0372 Mhar_0580

MPD:	MCP_2462(purP-1) MCP_2481(purP-2) MCP_2482(purP-3)

MEZ:	Mtc_2399(purP)

RCI:	RCIX2374(purP)

MJK:	N2V74_06085

MEAM:

MU439_05700 MU439_05705

MEAE:

QEN48_04455 QEN48_04460

TAR:	TALC_00174 TALC_01295

MAX:	MMALV_01620 MMALV_13860

MER:	MMINT_01670(purP) MMINT_02910

MEAR:

Mpt1_c09180(purP1) Mpt1_c13110(purP2)

MEUZ:

KRP56_00920 KRP56_06745

MARC:

AR505_0257 AR505_1667

SMR:

Smar_1525

IHO:	Igni_0442 Igni_0943

IIS:	EYM_01025 EYM_07900

IPC:	IPA_00050 IPA_03630

DKA:	DKAM_0529 DKAM_0531

DFD:	Desfe_0493 Desfe_0495

IAG:	Igag_0271 Igag_0273

PFM:	Pyrfu_0237 Pyrfu_0911

PDL:	Pyrde_0289 Pyrde_0749

PABI:

PABY_04650 PABY_24630

STO:	STK_02890(purP) STK_02900(purP)

SOH:	D1869_00985 D1869_00990

SSO:	SSO0239 SSO0241

SOL:	Ssol_1218 Ssol_1219

SSOA:

SULA_1258 SULA_1259

SSOL:

SULB_1259 SULB_1260

SSOF:

SULC_1257 SULC_1258

SSHI:

J5U23_00015 J5U23_00016

SCAS:

SACC_01530 SACC_01540

SAI:	Saci_0707 Saci_0708

SACN:

SacN8_03410(purP) SacN8_03415

SACR:

SacRon12I_03400(purP) SacRon12I_03405

SACS:

SUSAZ_03180 SUSAZ_03185

SIS:	LS215_2006 LS215_2007

SIA:	M1425_1897 M1425_1898

SIM:	M1627_1974 M1627_1975

SID:	M164_1904 M164_1905

SIY:	YG5714_1973 YG5714_1974

SIN:	YN1551_0946 YN1551_0947

SII:	LD85_2118 LD85_2119

SIH:	SiH_1834 SiH_1836

SIR:	SiRe_1754 SiRe_1756

SIC:	SiL_1747 SiL_1748

SULA:

BFU36_04630 BFU36_04635

SULE:

GFS03_10400 GFS03_10405

SULO:

GFS33_02215 GFS33_02220

SULL:

EWF20_02450 EWF20_02455

MSE:	Msed_0016 Msed_0017

MEMJ:

MJ1HA_0016 MJ1HA_0017

MCN:	Mcup_0016 Mcup_0017

MHK:	DFR87_07050 DFR87_07055

MPRU:

DFR88_06610 DFR88_06615

MTEN:

GWK48_05105 GWK48_05110

MJN:	MjAS7_0603 MjAS7_0604

AHO:	Ahos_0764 Ahos_0765

AMAN:

B6F84_07270 B6F84_07275

ABRI:

DFR85_07650 DFR85_07655

ASUL:

DFR86_06330 DFR86_06335

AAMB:

D1866_11640 D1866_11645

ACIH:

HS5_21380 HS5_21390

SACD:

HS1genome_0014 HS1genome_0015

SAZO:

D1868_01780 D1868_01785

CSTY:

KN1_18510 KN1_18520

STEP:

IC006_1811 IC006_1812

SMET:

RQ359_000029 RQ359_000030

PAI:	PAE0604 PAE0608

PIS:	Pisl_0094 Pisl_0095

PCL:	Pcal_0449 Pcal_2033

PAS:	Pars_2262 Pars_2265

PYR:	P186_2114 P186_2117

POG:	Pogu_2597 Pogu_2600

TNE:	Tneu_1918 Tneu_1919

PYW:	PYWP30_02163 PYWP30_02165

CMA:	Cmaq_0021 Cmaq_0022

TTN:	TTX_0542 TTX_0543

TUZ:	TUZN_0184 TUZN_0185

VDI:	Vdis_0677 Vdis_0678

VMO:	VMUT_1944 VMUT_1945

VSO:	Vsou_00990 Vsou_01000

FFO:	FFONT_0566 FFONT_0568

KCR:	Kcr_0400 Kcr_0401

NMR:	Nmar_0770 Nmar_0872

NIR:	NSED_04285 NSED_04890(purP)

NKR:	NKOR_04240 NKOR_04920(purP)

NID:	NPIRD3C_1324(purP) NPIRD3C_1455

NIN:	NADRNF5_1179(purP) NADRNF5_1308

NIW:	Nisw_00370 Nisw_00920

NCL:	C5F47_04390 C5F47_05060

NOX:	C5F49_04300 C5F49_04985

NUE:	C5F50_05790 C5F50_06470

NIP:	NsoK4_03660 NsoK4_04330

NZT:	NZOSNM25_001050 NZOSNM25_001167

NCT:	NMSP_0725(purP_1) NMSP_0856(purP_2)

NIC:	DSQ20_04355

NGA:	Ngar_c02760(purP1) Ngar_c27450(purP2)

NVN:	NVIE_014230(purP1) NVIE_015540(purP2)

NEV:	NTE_00824 NTE_02840

TAA:	NMY3_02655 NMY3_03715

NFN:	NFRAN_0263(purP) NFRAN_1004(purP)

NCV:	NCAV_0743(purP) NCAV_1281(purP)

NDV:	NDEV_1117(purP) NDEV_1244(purP)

CSY:	CENSYa_0242 CENSYa_0907

CSU:	CSUB_C0852 CSUB_C0853

NBV:	T478_0670 T478_0775

TAH:	SU86_001715 SU86_002275

NIU:	DSQ19_04515 DSQ19_05280

NCK:	QVH35_08620

CCAI:

NAS2_1505 NAS2_1506

BARB:

AOA66_0604(purP)

FLT:	Sv326_1155 Sv326_1156

» show all

Reference

1 [PMID:15623504]

Authors

Ownby K, Xu H, White RH

Title

A Methanocaldococcus jannaschii archaeal signature gene encodes for a 5-formaminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate synthetase. A new enzyme in purine biosynthesis.

Journal

J Biol Chem 280:10881-7 (2005)
DOI:10.1074/jbc.M413937200

Sequence

[mja:MJ_0136]

Reference

2 [PMID:18069798]

Authors

Zhang Y, White RH, Ealick SE

Title

Crystal structure and function of 5-formaminoimidazole-4-carboxamide ribonucleotide synthetase from Methanocaldococcus jannaschii.

Journal

Biochemistry 47:205-17 (2008)
DOI:10.1021/bi701406g