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| Status |
Public on Sep 28, 2012 |
| Title |
HudsonAlpha_ChipSeq_GM12878_TCF3_(SC-349)_PCR1x |
| Sample type |
SRA |
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| Source name |
GM12878
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| Organism |
Homo sapiens |
| Characteristics |
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
antibody antibodydescription: E2A.E12(V-18) is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the C-terminus of E2A.E12 of human origin. Recommended for detection of E12 and E47 of m, r and h origin by WB, IP, IF, IHC(P), ELISA and indirect FCM using PE (sc-3739)- or FITC (sc-2012)-conjugated goat anti-rabbit IgG. Antibody Target: TCF3
antibody targetdescription: Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
antibody vendorname: Santa Cruz Biotechnology
antibody vendorid: sc-349
controlid: SL1394,SL1395
labexpid: SL633,SL480
softwareversion: MACS
cell sex: F
antibody: TCF3_(SC-349)
antibody antibodydescription: E2A.E12(V-18) is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the C-terminus of E2A.E12 of human origin. Recommended for detection of E12 and E47 of m, r and h origin by WB, IP, IF, IHC(P), ELISA and indirect FCM using PE (sc-3739)- or FITC (sc-2012)-conjugated goat anti-rabbit IgG. Antibody Target: TCF3
antibody targetdescription: Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
antibody vendorname: Santa Cruz Biotechnology
antibody vendorid: sc-349
treatment: None
treatment description: No special treatment or protocol applies
protocol: PCR1x
protocol description: one 15-cycle round of PCR (Myers)
controlid: SL1394,SL1395
labexpid: SL633,SL480
replicate: 1,2
softwareversion: MACS
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| Biomaterial provider |
Coriell; http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM12878
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| Treatment protocol |
None
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| Growth protocol |
GM12878_protocol.pdf
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
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| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
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| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
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| Submission date |
Sep 28, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
[email protected]
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| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
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| Platform ID |
GPL9052 |
| Series (2) |
| GSE32465 |
Transcription Factor Binding Sites by ChIP-seq from ENCODE/HAIB |
| GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
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| Relations |
| SRA |
SRX190201 |
| BioSample |
SAMN01731630 |
| Named Annotation |
GSM1010745_hg19_wgEncodeHaibTfbsGm12878Tcf3Pcr1xRawRep1.bigWig |
| Named Annotation |
GSM1010745_hg19_wgEncodeHaibTfbsGm12878Tcf3Pcr1xRawRep2.bigWig |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM1010745_hg19_wgEncodeHaibTfbsGm12878Tcf3Pcr1xPkRep1.broadPeak.gz |
533.2 Kb |
(ftp)(http) |
BROADPEAK |
| GSM1010745_hg19_wgEncodeHaibTfbsGm12878Tcf3Pcr1xPkRep2.broadPeak.gz |
325.4 Kb |
(ftp)(http) |
BROADPEAK |
| GSM1010745_hg19_wgEncodeHaibTfbsGm12878Tcf3Pcr1xRawRep1.bigWig |
37.7 Mb |
(ftp)(http) |
BIGWIG |
| GSM1010745_hg19_wgEncodeHaibTfbsGm12878Tcf3Pcr1xRawRep2.bigWig |
19.3 Mb |
(ftp)(http) |
BIGWIG |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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