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| Status |
Public on Sep 29, 2011 |
| Title |
HudsonAlpha_ChipSeq_GM12878_POU2F2_PCR1x |
| Sample type |
SRA |
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| Source name |
GM12878
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| Organism |
Homo sapiens |
| Characteristics |
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell description: B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
antibody antibodydescription: Oct-2(C-20) is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the C-terminus of Oct-2 of human origin. Recommended for detection of Oct-2 of m, r and h origin by WB, IP, IF, IHC(P) and ELISA. Antibody Target: POU2F2
antibody targetdescription: Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
antibody vendorname: Santa Cruz Biotechnology
antibody vendorid: sc-233
protocol description: one 15-cycle round of PCR (Myers)
controlid: SL1395,SL516
labexpid: SL648,SL851,SL614
labversion: SL648,SL614
replicate: 1,3,2
softwareversion: MACS
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-233
treatment: None
treatment description: No special treatment or protocol applies
protocol: PCR1x
protocol description: 1-cycle of PCR (Myers)
controlid: SL516
labexpid: SL614
replicate: 1
replicate description: tier 1
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| Biomaterial provider |
Coriell; http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM12878
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| Treatment protocol |
None
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| Growth protocol |
GM12878_protocol.pdf
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
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| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
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| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
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| Submission date |
Sep 28, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
[email protected]
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| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
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| Platform ID |
GPL9052 |
| Series (2) |
| GSE32465 |
Transcription Factor Binding Sites by ChIP-seq from ENCODE/HAIB |
| GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
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| Relations |
| SRA |
SRX100579 |
| BioSample |
SAMN00738496 |
| Named Annotation |
GSM803534_hg19_wgEncodeHaibTfbsGm12878Pou2f2Pcr1xRawRep1.bigWig |
| Named Annotation |
GSM803534_hg19_wgEncodeHaibTfbsGm12878Pou2f2Pcr1xRawRep2.bigWig |
| Named Annotation |
GSM803534_hg19_wgEncodeHaibTfbsGm12878Pou2f2Pcr1xRawRep3.bigWig |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM803534_hg19_wgEncodeHaibTfbsGm12878Pou2f2Pcr1xPkRep1.broadPeak.gz |
348.8 Kb |
(ftp)(http) |
BROADPEAK |
| GSM803534_hg19_wgEncodeHaibTfbsGm12878Pou2f2Pcr1xPkRep2.broadPeak.gz |
652.7 Kb |
(ftp)(http) |
BROADPEAK |
| GSM803534_hg19_wgEncodeHaibTfbsGm12878Pou2f2Pcr1xPkRep3.broadPeak.gz |
238.8 Kb |
(ftp)(http) |
BROADPEAK |
| GSM803534_hg19_wgEncodeHaibTfbsGm12878Pou2f2Pcr1xRawRep1.bigWig |
25.8 Mb |
(ftp)(http) |
BIGWIG |
| GSM803534_hg19_wgEncodeHaibTfbsGm12878Pou2f2Pcr1xRawRep2.bigWig |
90.4 Mb |
(ftp)(http) |
BIGWIG |
| GSM803534_hg19_wgEncodeHaibTfbsGm12878Pou2f2Pcr1xRawRep3.bigWig |
11.2 Mb |
(ftp)(http) |
BIGWIG |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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