Electroporated recombinant proteins as tools for in vivo functional complementation, imaging and chemical biology (Q83231110)

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25 April 2020

title

Electroporated recombinant proteins as tools for in vivo functional complementation, imaging and chemical biology (English)

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25 April 2020

Valentina Piano

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Marchel Stuiver

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Yaowen Wu

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Andrea Musacchio

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Stefano Maffini

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author name string

Soumitra Polley

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Stephanie Voss

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Giuseppe Ciossani

6

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Katharina Overlack

7

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Beate Voss

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Sabine Wohlgemuth

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Arsen Petrovic

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Philipp Selenko

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language of work or name

English

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publication date

16 July 2019

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25 April 2020

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25 April 2020

volume

8

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Penetration and entrapment of large particles in erythrocytes by electrical breakdown techniques

25 April 2020

cites work

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12 December 2020

Electroporation-mediated uptake of proteins into mammalian cells

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12 December 2020

Transfer of monoclonal antibodies into mammalian cells by electroporation

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Magic bullets for protein kinases

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Correcting improper chromosome-spindle attachments during cell division

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Structure-based assembly of protein complexes in yeast

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Molecular organization of the Ndc80 complex, an essential kinetochore component

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Development of farnesyl transferase inhibitors: a review

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S-trityl-L-cysteine is a reversible, tight binding inhibitor of the human kinesin Eg5 that specifically blocks mitotic progression.

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Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore

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The social network of a cell: recent advances in interactome mapping

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A new mechanism controlling kinetochore-microtubule interactions revealed by comparison of two dynein-targeting components: SPDL-1 and the Rod/Zwilch/Zw10 complex

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High-resolution multi-dimensional NMR spectroscopy of proteins in human cells.

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Site-specific protein labeling via sortase-mediated transpeptidation

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Super-resolution fluorescence microscopy

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12 December 2020

Observation of NMR signals from proteins introduced into living mammalian cells by reversible membrane permeabilization using a pore-forming toxin, streptolysin O.

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12 December 2020

Mammalian cell transfection: the present and the future

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12 December 2020

The MIS12 complex is a protein interaction hub for outer kinetochore assembly

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12 December 2020

Protein interaction domain mapping of human kinetochore protein Blinkin reveals a consensus motif for binding of spindle assembly checkpoint proteins Bub1 and BubR1

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12 December 2020

The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis

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12 December 2020

Microinjection of protein samples

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12 December 2020

Interactome networks and human disease

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12 December 2020

Investigating a macromolecular complex: the toolkit of methods

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12 December 2020

MultiBac: expanding the research toolbox for multiprotein complexes.

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12 December 2020

Designer proteins: applications of genetic code expansion in cell biology

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A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B

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Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells

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12 December 2020

Synthetic biology in mammalian cells: next generation research tools and therapeutics

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12 December 2020

Modular assembly of RWD domains on the Mis12 complex underlies outer kinetochore organization

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12 December 2020

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

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12 December 2020

KRas localizes to the plasma membrane by spatial cycles of solubilization, trapping and vesicular transport

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12 December 2020

Intracellular delivery of protein and peptide therapeutics

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12 December 2020

Promises and pitfalls of intracellular delivery of proteins

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12 December 2020

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

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12 December 2020

Multiple assembly mechanisms anchor the KMN spindle checkpoint platform at human mitotic kinetochores

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12 December 2020

A molecular basis for the differential roles of Bub1 and BubR1 in the spindle assembly checkpoint

1 reference

12 December 2020

Regulated eukaryotic DNA replication origin firing with purified proteins

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12 December 2020

Ras moves to stay in place

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12 December 2020

Preventing farnesylation of the dynein adaptor Spindly contributes to the mitotic defects caused by farnesyltransferase inhibitors

1 reference

12 December 2020

A novel role of farnesylation in targeting a mitotic checkpoint protein, human Spindly, to kinetochores

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12 December 2020

Efficient intracellular delivery of native proteins

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12 December 2020

Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection.

1 reference

12 December 2020

Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing

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12 December 2020

Adaptive changes in the kinetochore architecture facilitate proper spindle assembly

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12 December 2020

Genetic code expansion enabled site-specific dual-color protein labeling: superresolution microscopy and beyond

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12 December 2020

The Molecular Biology of Spindle Assembly Checkpoint Signaling Dynamics

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12 December 2020

Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells

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12 December 2020

Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint

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12 December 2020

Structural disorder of monomeric α-synuclein persists in mammalian cells.

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12 December 2020

biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes

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12 December 2020

Insights from biochemical reconstitution into the architecture of human kinetochores.

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12 December 2020

Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores

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12 December 2020

Spatiotemporal imaging of small GTPases activity in live cells

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12 December 2020

Labeling proteins inside living cells using external fluorophores for microscopy

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12 December 2020

Molecular basis of outer kinetochore assembly on CENP-T.

1 reference

12 December 2020

Basis of catalytic assembly of the mitotic checkpoint complex

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12 December 2020

A Molecular View of Kinetochore Assembly and Function

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12 December 2020

Structure of the RZZ complex and molecular basis of its interaction with Spindly.

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12 December 2020

A Method for the Acute and Rapid Degradation of Endogenous Proteins.

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12 December 2020

The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases.

1 reference

12 December 2020

The kinetochore-microtubule interface at a glance

1 reference

12 December 2020

Reconstitution of a 26-Subunit Human Kinetochore Reveals Cooperative Microtubule Binding by CENP-OPQUR and NDC80

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12 December 2020

Multiple phosphorylations control recruitment of the KMN network onto kinetochores

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12 December 2020

A Review on Electroporation-Based Intracellular Delivery

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12 December 2020

A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall

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12 December 2020

Identifiers

DOI

10.7554/ELIFE.48287

1 reference

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25 April 2020

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25 April 2020

PubMed publication ID

31310234

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