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filingDate 1993-09-29^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1997-02-11^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49a5a1000ecf2ab2d58654076736a3b6
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publicationDate 1997-02-11^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-5602207-A
titleOfInvention Support and method for immobilizing polypeptides
abstract A solid support and method for immobilization and sequence analysis of polypeptides. In one aspect, the invention is directed to a method for immobilizing a polypeptide on a solid support. In the method, a solid support having surface-attached carboxylic acid groups is reacted with an isoxazolium salt to form an activated support. After the activated support has been washed to remove residual isoxazolium salt and base, the support is dried. The dried support is then contacted with a polypeptide under conditions effective to bind the polypeptide covalently to the support. The immobilized polypeptide can be conveniently sequenced by N- and C-terminal sequencing methods.
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