| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efda7341819f1a85d7f2fe104cd8a26f |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08G83-004
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-645
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-6898 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87 |
| filingDate |
2001-04-03^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2007-03-20^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9252779f39fcdab067befaa594e82ddf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9071c0af41d76898d97510046cf49167
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b2c976259f8193386ec7fb5039259c23 |
| publicationDate |
2007-03-20^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-7192744-B2 |
| titleOfInvention |
Targeted transfection of cells using a biotinylated dendrimer |
| abstract |
The present invention relates to processes for the targeted transfection of cells, compositions which may be used for such processes, and corresponding pharmaceutical compositions for gene therapy. In particular, the invention relates to a process for introducing nucleic acid into cells comprising the steps of (a) mixing a nucleic acid with a dendrimer, in which a proportion of the dendrimer molecules are biotinylated; (b) mixing the resulting complex of nucleic acid and dendrimer with a second complex consisting of avidin or streptavidin and a biotinylated target-specific binding molecule; and (c) incubating the complex formed in step (b) with cells. Dendrimers which are well suited to the present invention include, for example, partially solvolysed polyamidoamine (PAMAM) dendrimers. Target-specific binding molecules are, in particular, cell-type-specific markers of the cell surface of the target cells. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8202544-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010113695-A1 |
| priorityDate |
2000-04-05^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |