http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016056566-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ee1c19da359446fb5c4f0458a57b783d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-645 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-145 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-01001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01027 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 2015-10-06^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c266acf6a62dec28746e4751bebdc6f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44398eaef47ac750bf0d874c2c1d9a8b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e4430e746d6e545a967a60b1b37b4dab |
| publicationDate | 2016-04-14^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2016056566-A1 |
| titleOfInvention | Transformant and method for producing same, and method for producing lactic acid |
| abstract | Provided are: a transformant that is capable of producing lactic acid at a high productivity without requiring neutralization with an alkaline agent, and has excellent lactic acid production ability and excellent proliferation potency; a method for producing the transformant; and a method for producing lactic acid using the transformant. A transformant characterized in that: the host thereof is Schizosaccharomyces pombe; 3-5 copies of a human-derived lactate dehydrogenase gene are integrated thereinto; and some genes belonging to a gene group encoding pyruvate decarboxylase of the Schizosaccharomyces pombe host are deleted or deactivated therein. A transformant characterized in that: when the transformant is inoculated into 100 ml of a liquid medium in a 500 ml Sakaguchi flask, said liquid medium comprising 1% of yeast extract, 2% of peptone and 6% of glucose, at an initial cell concentration of 0.04 g (dcw)/l and then cultured for 20 hours at a temperature of 32°C under shaking at 110 rpm and a shaking width of 7 cm, the cell concentration of the liquid culture is 4.0 g (dcw)/l or greater; and, when the cells obtained by culturing for 20 hours under the same conditions as described above are inoculated into a 11.1% aqueous glucose solution at an initial cell concentration of 36 g (in terms of dry cells)/l and then fermented for 3 hours at a temperature of 32°C under shaking at 110 rpm and a shaking width of 7 cm, the lactic acid concentration of the fermented liquor is 80 g/l or greater. A method for producing lactic acid using any of the aforesaid transformants. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7210577-B2 |
| priorityDate | 2014-10-10^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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