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MyoD stimulates delta-1 transcription and triggers notch signaling in the Xenopus gastrula.

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  • 1. Friedrich Miescher Laboratory, MPG, Spemannstrasse 37-39, D-72076 Tübingen, Germany.
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    • Wittenberger T 1
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The EMBO Journal, 01 Apr 1999, 18(7):1915-1922
https://doi.org/10.1093/emboj/18.7.1915 PMID: 10202155 PMCID: PMC1171277

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Abstract 

The Notch signaling cascade is involved in many developmental decisions, a paradigm of which has been the selection between epidermal and neural cell fates in both invertebrates and vertebrates. Notch has also been implicated as a regulator of myogenesis, although its precise function there has remained controversial. Here we show that the muscle-determining factor MyoD is a direct, positive regulator of the Notch ligand Delta-1 in prospective myoblasts of the pre-involuted mesoderm in Xenopus gastrulae. Injection of a dominant MyoD repressor variant ablates mesodermal Delta-1 expression in vivo. Furthermore, MyoD-dependent Delta-1 induction is sufficient to activate transcription from promoters of E(spl)-related genes in a Notch-dependent manner. These results indicate that a hallmark of neural cell fate determination, i.e. the feedback loop between differentiation promoting basic helix-loop-helix proteins and the Notch regulatory circuitry, is conserved in myogenesis, supporting a direct involvement of Notch in muscle determination.

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EMBO J. 1999 Apr 1; 18(7): 1915–1922.
PMCID: PMC1171277
PMID: 10202155

MyoD stimulates delta-1 transcription and triggers notch signaling in the Xenopus gastrula.

T Wittenberger, O C Steinbach, A Authaler, R Kopan, and R A Rupp
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Abstract

The Notch signaling cascade is involved in many developmental decisions, a paradigm of which has been the selection between epidermal and neural cell fates in both invertebrates and vertebrates. Notch has also been implicated as a regulator of myogenesis, although its precise function there has remained controversial. Here we show that the muscle-determining factor MyoD is a direct, positive regulator of the Notch ligand Delta-1 in prospective myoblasts of the pre-involuted mesoderm in Xenopus gastrulae. Injection of a dominant MyoD repressor variant ablates mesodermal Delta-1 expression in vivo. Furthermore, MyoD-dependent Delta-1 induction is sufficient to activate transcription from promoters of E(spl)-related genes in a Notch-dependent manner. These results indicate that a hallmark of neural cell fate determination, i.e. the feedback loop between differentiation promoting basic helix-loop-helix proteins and the Notch regulatory circuitry, is conserved in myogenesis, supporting a direct involvement of Notch in muscle determination.

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Selected References

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