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Adenovirus as an expression vector in muscle cells in vivo.

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Affiliations

  • 1. Laboratoire de Génétique Moléculaire des Eucaryotes, U184, Institut de Chimie Biologique, Strasbourg, France.
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    • Quantin B 1
    (1 author)

Proceedings of the National Academy of Sciences of the United States of America, 01 Apr 1992, 89(7):2581-2584
https://doi.org/10.1073/pnas.89.7.2581 PMID: 1557362 PMCID: PMC48705

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Abstract 

Attempting gene transfer in muscle raises difficult problems: the nuclei of mature muscle fibers do not undergo division, thus excluding strategies involving replicative integration of exogenous DNA. As adenovirus has been reported to be an efficient vector for the transfer of an enzyme encoding gene in mice, we decided to explore its potential for muscle cells. Advantages of adenovirus vectors are their independence of host cell replication, broad host range, and potential capacity for large foreign DNA inserts. We constructed a recombinant adenovirus containing the beta-galactosidase reporter gene under the control of muscle-specific regulatory sequences. This recombinant virus was able to direct expression of the beta-galactosidase in myotubes in vitro. We report its in vivo expression in mouse muscles up to 75 days after infection. The efficiency and stability of expression we obtained compare very favorably with other strategies proposed for gene or myoblast transfer in muscle in vivo.

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Proc Natl Acad Sci U S A. 1992 Apr 1; 89(7): 2581–2584.
PMCID: PMC48705
PMID: 1557362

Adenovirus as an expression vector in muscle cells in vivo.

B Quantin, L D Perricaudet, S Tajbakhsh, and J L Mandel
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Abstract

Attempting gene transfer in muscle raises difficult problems: the nuclei of mature muscle fibers do not undergo division, thus excluding strategies involving replicative integration of exogenous DNA. As adenovirus has been reported to be an efficient vector for the transfer of an enzyme encoding gene in mice, we decided to explore its potential for muscle cells. Advantages of adenovirus vectors are their independence of host cell replication, broad host range, and potential capacity for large foreign DNA inserts. We constructed a recombinant adenovirus containing the beta-galactosidase reporter gene under the control of muscle-specific regulatory sequences. This recombinant virus was able to direct expression of the beta-galactosidase in myotubes in vitro. We report its in vivo expression in mouse muscles up to 75 days after infection. The efficiency and stability of expression we obtained compare very favorably with other strategies proposed for gene or myoblast transfer in muscle in vivo.

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Selected References

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