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A 3.5 genome equivalent multi access YAC library: construction, characterisation, screening and storage.

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  • 1. ICI Diagnostics, Northwich, Cheshire, UK.
      Authors
    • Anand R 1
    (1 author)

Nucleic Acids Research, 01 Apr 1990, 18(8):1951-1956
https://doi.org/10.1093/nar/18.8.1951 PMID: 2186372 PMCID: PMC330667

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Abstract 

The construction of a yeast artificial chromosome (YAC) primary gridded library of 35,000 clones from human lymphoblastoid (48,XXXX) cell line DNA is described. The average YAC size is approximately 350kb representing a greater than 3.5 times coverage of the genome. The library is stored at -70 degrees C as gridded clones on nylon filters impregnated with 20% glycerol and as glycerol suspensions of individual clones in microtitre plates providing a prolonged multi-user potential. To date we have used 14 single copy probes to screen this library by colony hybridisation as well as PCR and have isolated between 1 and 5 YAC clones for every probe.

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Nucleic Acids Res. 1990 Apr 25; 18(8): 1951–1956.
PMCID: PMC330667
PMID: 2186372

A 3.5 genome equivalent multi access YAC library: construction, characterisation, screening and storage.

R Anand, J H Riley, R Butler, J C Smith, and A F Markham
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Abstract

The construction of a yeast artificial chromosome (YAC) primary gridded library of 35,000 clones from human lymphoblastoid (48,XXXX) cell line DNA is described. The average YAC size is approximately 350kb representing a greater than 3.5 times coverage of the genome. The library is stored at -70 degrees C as gridded clones on nylon filters impregnated with 20% glycerol and as glycerol suspensions of individual clones in microtitre plates providing a prolonged multi-user potential. To date we have used 14 single copy probes to screen this library by colony hybridisation as well as PCR and have isolated between 1 and 5 YAC clones for every probe.

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Selected References

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