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Transforming growth factor alpha: an aromatic side chain at position 38 is essential for biological activity.

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  • 1. Laboratory of Chemoprevention, National Cancer Institute, Bethesda, Maryland 20892.
      Authors
    • Lazar E 1
    (1 author)

Molecular and Cellular Biology, 01 Feb 1989, 9(2):860-864
https://doi.org/10.1128/mcb.9.2.860-864.1989 PMID: 2710128 PMCID: PMC362669

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Abstract 

Site-directed mutagenesis has been performed in the human transforming growth factor alpha gene. When tyrosine 38 is mutated into phenylalanine or tryptophane, biological activity is retained. In contrast, other alterations between cysteine 34 and cysteine 43 and disruption of disulfide bonds 8 to 21 and 34 to 43 resulted in loss of activities. The presence of an aromatic side chain at position 38 of transforming growth factor alpha seems to be essential for its activity.

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Mol Cell Biol. 1989 Feb; 9(2): 860–864.
PMCID: PMC362669
PMID: 2710128

Transforming growth factor alpha: an aromatic side chain at position 38 is essential for biological activity.

E Lazar, E Vicenzi, E Van Obberghen-Schilling, B Wolff, S Dalton, S Watanabe, and M B Sporn
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Abstract

Site-directed mutagenesis has been performed in the human transforming growth factor alpha gene. When tyrosine 38 is mutated into phenylalanine or tryptophane, biological activity is retained. In contrast, other alterations between cysteine 34 and cysteine 43 and disruption of disulfide bonds 8 to 21 and 34 to 43 resulted in loss of activities. The presence of an aromatic side chain at position 38 of transforming growth factor alpha seems to be essential for its activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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