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Substructure of inner dynein arms, radial spokes, and the central pair/projection complex of cilia and flagella.

The Journal of Cell Biology, 01 Jun 1985, 100(6):2008-2018
https://doi.org/10.1083/jcb.100.6.2008 PMID: 2860115 PMCID: PMC2113608

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Abstract 

The substructure of the components of the axoneme interior--the inner dynein arms, the radial spokes, and the central pair/projection complex--was analyzed for Chlamydomonas. Tetrahymena, Strongelocentrotus, and Mnemiopsis using the quick-freeze, deep-etch technique. The inner arms are shown to resemble the outer arms in overall molecular organization, but they are disposed differently on the microtubule and have two distinct morphologies--dyads with two heads and triads with three. The dyads associate with spokes S3 and S2; the triads associate with S1. The spokes form a three-start right-handed helix with a 288-nm rise; the central pair makes a shallow left-handed twist. The spoke heads are shown to be made up of four major subunits; two bind to the spoke shaft and two bind to a pair of central-sheath projections.

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J Cell Biol. 1985 Jun 1; 100(6): 2008–2018.
PMCID: PMC2113608
PMID: 2860115

Substructure of inner dynein arms, radial spokes, and the central pair/projection complex of cilia and flagella

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Abstract

The substructure of the components of the axoneme interior--the inner dynein arms, the radial spokes, and the central pair/projection complex- -was analyzed for Chlamydomonas. Tetrahymena, Strongelocentrotus, and Mnemiopsis using the quick-freeze, deep-etch technique. The inner arms are shown to resemble the outer arms in overall molecular organization, but they are disposed differently on the microtubule and have two distinct morphologies--dyads with two heads and triads with three. The dyads associate with spokes S3 and S2; the triads associate with S1. The spokes form a three-start right-handed helix with a 288-nm rise; the central pair makes a shallow left-handed twist. The spoke heads are shown to be made up of four major subunits; two bind to the spoke shaft and two bind to a pair of central-sheath projections.

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Selected References

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NIGMS NIH HHS (1)