Abstract

Introduction

Damage to the prefrontal cortex (PFC) in humans impairs behavioral flexibility (Milner et al., 1985; Colvin et al., 2001), decision making (Bechara et al., 2000; Manes et al., 2002), and planning (Bechara et al., 1996; Muller et al., 2002). In rats, the medial PFC (mPFC) is homologous to the dorsolateral PFC in primates (Vogt et al., 2004). Lesions in this area typically disrupt executive functions, including response inhibition (Li and Shao, 1998; Salazar et al., 2004), temporal organization of behavior (Hannesson et al., 2004; Hata and Okaichi, 2004), and working memory (Shaw and Aggleton, 1993; Joel et al., 1997; Kesner, 2000). Deficits in working memory, associated with prefrontal cortical dysfunction, are a hallmark of several neuropsychiatric disorders, including depression, Parkinson's disease, and schizophrenia (Lewis et al., 2004; Monchi et al., 2004; Rogers et al., 2004; Shad et al., 2004). Both stress and hypersecretion of corticosteroids have been causally implicated in depression and schizophrenia (Altamura et al., 1999; Corcoran et al., 2002; Barden, 2004; Ryan et al., 2004). These observations complement recent evidence that stress per se (Mizoguchi et al., 2000) or adrenocorticosteroids (Coburn-Litvak et al., 2003; Roozendaal et al., 2004) can profoundly influence PFC-dependent cognitive processes and that impaired glucocorticoid-negative feedback on the hypothalamo-pituitary-adrenal (HPA) axis may involve the PFC and hippocampus (Diorio et al., 1993; Sullivan and Gratton, 2002; Mizoguchi et al., 2003).

Corticosteroid actions in the brain are mediated by mineralocorticoid (MR) and glucocorticoid (GR) receptors (Reul and de Kloet, 1985). In addition to the hippocampus, a region characterized by a high density of MR and GR and with an established role in the physiological and behavioral adaptation to stress (de Kloet et al., 1998; Reul et al., 2000), the mPFC has a rich population of corticosteroid receptors (Chao et al., 1989; Herman, 1993; Patel et al., 2000), which become activated during stress (Cullinan et al., 1995; Figueiredo et al., 2003). Changes in neuronal morphology and survival are thought to, at least partly, underpin corticosteroid-induced alterations in hippocampal function (Sousa and Almeida, 2002). Recent studies from two laboratories have demonstrated that chronic treatments with either corticosterone or restraint stress induce a remodeling of apical dendrites of layer II/III pyramidal neurons in the mPFC, such that there is greater proximal branching, whereas spine density is reduced (Wellman, 2001; Cook and Wellman, 2004; Radley et al., 2004, 2005). Although alterations of the corticosteroid milieu have also been reported to result in impaired spatial working memory, as measured in the T-maze (Mizoguchi et al., 2000, 2004) and Y-maze (Coburn-Litvak et al., 2003), a direct structure-function relationship has not yet been established. The present study was intended to fill this gap by parallel evaluation of cognitive performance and PFC morphology. Briefly, a detailed stereological analysis was conducted on the mPFC of rats whose working memory and behavioral flexibility had been tested during exposure to different corticosteroid environments. The latter were induced by adrenalectomy (leaving the animals corticosteroid-free) or by chronic treatment with either an endogenous corticosteroid [corticosterone (CORT)] or the synthetic GR-selective agonist dexamethasone (DEX).

Materials and Methods

Results

Biometric parameters and hormonal determinations

The efficacy of the various hormonal manipulations was proven by their effects on body weight (Fig. 1A). Repeated-measures ANOVA revealed a significant effect of both time after start of treatment (F_(5,180) = 949.004; p < 0.001) and experimental group (F_(3,36) = 177.183; p < 0.001), as well as an interaction between these two factors (F_(15,180) = 667.651; p < 0.001). Compared with controls (and overall), both ADX and CORT-treatment groups resulted in a decreased body weight gain, which was more prominent in the latter; in contrast, DEX-treated rats showed a net weight loss.

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Figure 1.

Biometric parameters and hormonal determinations. A, Body weights over the course of the study. B, Thymus/body weight ratios. C, Adrenal weights. D, Serum corticosterone levels with respect to time of day (left, 1 h after lights on, 9:00 A.M.; middle, middle of the daily light/dark cycle, 3:00P.M.; right, 1 h after lights out, 9:00 P.M.) after 4 weeks of exposure to the various treatments. CORT and DEX were injected 1 h before lights off; CON and ADX animals received vehicle injections. ^*p < 0.05 versus all other groups; ^#p < 0.05 versus CON and versus ADX; ^##p < 0.05 versus CON and versus CORT; ⁺⁺p < 0.05 versus CON. Error bars represent SEM.

ANOVA revealed a significant effect of treatment on thymus/body weight ratio at the time of autopsy (F_(3,36) = 214.688; p < 0.001). Compared with controls, the thymus/body weight ratio was significantly increased in ADX animals (p < 0.001) and significantly decreased (p < 0.001) in rats exposed to either CORT (p < 0.001) or DEX (Fig. 1B).

ANOVA also revealed a significant effect of treatment on adrenal weight (F_(2,12) = 46.906; p < 0.001). Compared with controls, adrenal weight was significantly decreased in CORT- (41%; p < 0.001) and DEX- (55%; p < 0.001) treated rats; adrenal weights did not differ between the latter groups (Fig. 1C).

Repeated-measures ANOVA showed that all treatments induced significant changes in plasma CORT levels (F_(3,36) = 208.625; p < 0.001) (Fig. 1D). Although CORT was undetectable in the serum of both ADX and DEX-treated animals (as expected), animals treated with CORT displayed five to eight times higher circulating levels of the hormone (day and night), compared with controls (CORT vs CON, vs DEX, and vs ADX, p < 0.001; CON vs DEX, p = 0.027, vs ADX, p = 0.01) (Fig 1D).

Behavioral data

Spatial working memory task

The analysis of the distance-swum learning curves in the spatial working memory task (Fig. 2B) revealed a significant effect of treatment (F_(3,36) = 3.074; p = 0.04). DEX-treated rats showed impaired learning ability, swimming longer distances on the second (55% more), third (92% more), and fourth (74% more) trials compared with controls (p = 0.022) but not ADX rats, which nevertheless showed learning curves similar to those observed for controls. The performance of CORT-treated rats did not differ significantly from any of the other experimental groups.

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Figure 2.

Behavioral data. A, Schematic representation of the experimental procedure. The position of the platform on each day is also indicated (above). Note that, during the 3 d allowed for memory consolidation (days 4-6), the platform was kept at the same position. B, Learning curve in the working memory task. The higher distances swum by the DEX-treated rats is easily appreciated. C, Results from the reverse task experiment: average distance swum on the four trials in each imaginary quadrant is given as a percentage of the total distance swum. ^*p < 0.05 versus all other groups; ^#p < 0.05 versus CON and versus ADX; ⁺⁺p < 0.05 versus CON. Error bars represent SEM.

Reverse learning task

On the second day of the reference memory task (day 5), DEX-treated rats were still performing worse than both CON and CORT-treated animals, whereas ADX were not different from any other group (CON, 245 ± 33 cm; CORT, 251 ± 27 cm; DEX, 420 ± 50 cm; ADX, 356 ± 55; F_(3,36) = 3.87; p = 0.016). The platform was kept in the same position for another day (day 6) to ensure that all animals had learned the platform position and were performing equally well on this test.

All rats had learned the platform position equally well by the day before the reverse task (day 6) was performed: ANOVA on distance swum revealed no between-group differences (CON, 243 ± 31 cm; CORT, 208 ± 30; DEX, 295 ± 42 cm; ADX, 260 ± 46 cm; F_(3,36) = 1.264; p = 0.301).

Analysis of the percentage of distance swum in each quadrant during the course of the reverse learning test (Fig. 2C) failed to reveal differences between treatment groups on either of the neutral quadrants (S, F_(3,36) = 0.908, p = 0.447; N, F_(3,36) = 0.500, p = 0.685). However, a significant effect of treatment was found for quadrants E (position of platform during preceding 3 d) and W (new location of the platform) (E, F_(3,36) = 13.143, p < 0.001; W, F_(3,36) = 14.172, p < 0.001). Controls promptly learned the location of the new platform, compared with all of the other groups, as shown by the simultaneous decreased distance swum in quadrant E (55% less vs ADX, p = 0.0014; 60% less vs CORT, p = 0.002; 70% less vs DEX, p < 0.001) and increased distance in quadrant W (ADX, p = 0.027; CORT, p = 0.015; DEX, p < 0.001). In contrast, DEX-treated rats were more persistent in searching the platform on its previous location (quadrant E) compared with controls (p < 0.001) and ADX (p = 0.024); consequently, they swam lesser distances than any other group on the newer location, W (vs CON, p < 0.001; vs ADX, p = 0.006; vs CORT, p = 0.011).

Volumes and neuronal numbers

The shrinkage factor was calculated as 1.08, 1.07, 1.04, and 1.11, respectively, for controls, CORT, DEX, and ADX treatment groups. The slight variations found in DEX- and ADX-treated groups are consistent with the expected reduced (DEX-induced) or increased (ADX-induced) water content of brain tissue.

Cingulate cortex

Volumes of layer I of the anterior cingulate cortex (ACC) were not significantly different across the four treatment groups (F_(3,16) = 0.164; p = 0.919) (Fig. 3B). In contrast, there was a significant effect of treatment on the volume of layer II (F_(3,16) = 23.585; p < 0.001) (Fig. 3C) and layers III-VI (F_(3,16) = 16.054; p < 0.001) (Fig. 3D). In CORT- and DEX-treated rats, the volumes of these layers were significantly reduced (between 10 and 25%) compared with those of ADX (layer II CORT, p < 0.001, DEX, p < 0.001; layer III-VI CORT, p = 0.001, DEX, p < 0.001) and control animals (layer II CORT, p < 0.001, DEX, p < 0.001; layer III-VI CORT, p = 0.009; DEX, p = 0.002). No significant differences were found between the volumes of ADX and control rats or between the volumes of CORT- and DEX-treated animals.

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Figure 3.

Stereology estimations. A, Boundaries between different regions and layers, traced over brain slices stained with Giemsa (left to right) at +2.2, +0.7, and -3.8 cm from bregma, respectively (magnification, 7.9×). B-D, Average volumes of layers I, II, and III-VI of the five regions studied. E, F, Estimated number of neurons in layers II and III-VI of the five regions studied. Note the different (and broken) scales used and that, with the exception of the numerical values for the IL and PL, which should be read off against the left axis, all other values should be read off against the right one. ^*p < 0.05 versus every other group; ^#p < 0.05 versus CON and versus ADX; ⁺p < 0.05 versus ADX; ⁺⁺p < 0.05 versus CON. Error bars represent SEM.

The number of neurons on layer II was significantly influenced by treatment (F_(3,16) = 6.925; p = 0.003) (Fig. 3E). Post hoc comparisons revealed that cell numbers in layer II of ACC in DEX-treated rats were significantly reduced (by 20% vs CON, p = 0.006; 16% vs CORT, p = 0.043; 20% vs ADX, p = 0.006). No differences were observed in the number of neurons in layers III-VI (F_(3,16) = 1.209; p = 0.338). (Fig. 3F)

Prelimbic cortex

Volumetric analysis in the prelimbic cortex revealed a significant effect of treatment on all layers (layer I, F_(3,16) = 3.519, p = 0.039; layer II, F_(3,16) = 8.586, p = 0.001; layers III-IV, F_(3,16) = 4.757, p = 0.015) (Fig. 3B-D). The volume of layer II in DEX-treated rats was reduced by some 18% versus controls (p = 0.008) and ADX animals (p = 0.003), whereas this measure was 15% less in CORT-treated versus ADX rats (p = 0.025); the latter did not differ significantly from control values. In both layers I and III-VI, significant differences were only detectable between the volumes of DEX-treated and ADX rats (layer I, p = 0.039; layers III-VI, p = 0.012). Again, no significant differences were found between the volumes of ADX and control rats or between the volumes of CORT- and DEX-treated animals.

ANOVA revealed an effect of treatment in neuronal numbers in layer II of the prelimbic cortex (F_(3,16) = 3.948; p = 0.028). A significantly smaller number of neurons (23% fewer, p = 0.002) was observed in layer II of DEX-treated versus control rats; neuron numbers in this layer did not otherwise differ between the various experimental groups (Fig. 3E). Treatment per se did not affect the number of neurons in layers III-VI of the prelimbic cortex (F_(3,16) = 0.780; p = 0.522) (Fig. 3F).

Infralimbic cortex

Treatment proved to be a significant factor with respect to the volumes of layers I and II of the infralimbic cortex (layer I, F_(3,16) = 4.673, p = 0.016; layer II, F_(3,16) = 7.047, p = 0.003) (Fig. 3B,C). Within the most superficial layer, the only significant difference found was between DEX-treated and ADX animals (p = 0.032). Conversely, the volume of layer II in both DEX- and CORT-treated rats was reduced compared with that in controls (DEX, p = 0.046; CORT, p = 0.041) and ADX rats (DEX, p = 0.015; CORT, p = 0.013). The volumes of layers III-VI did not differ significantly in any of the experimental groups (F_(3,16) = 2.765; p = 0.076) (Fig. 3D).

The number of neurons in layer II of DEX-treated rats was significantly reduced (F_(3,16) = 4.005; p = 0.026) compared with controls (23% lower, p = 0.041) and ADX animals (12% lower, p = 0.049) (Fig. 3E). No other differences in the number of layer II neurons were observed, regardless of treatments. Neuronal numbers of layers III-VI were similar between all groups (F_(3,16) = 0.314; p = 0.815) (Fig. 3F).

Retrosplenial cortex

Volumes of layers I, II, and II-VI of the RSC did not differ significantly across the four treatment groups (layer I, F_(3,16) = 1.106, p = 0.37; layer II, F_(3,16) = 1.734, p = 0.20; layers III-VI, F_(3,16) = 2.045, p = 0.148) (Fig. 3B-D). Likewise, no significant effects of treatment were found with respect to neuronal numbers in layers II (F_(3,16) = 0.164; p = 0.91) and III-VI (F_(3,16) = 0.466; p = 0.710) of the RSC (Fig. 3E,F).

Motor cortex

The volumes of MC layers I, II, and III-VI did not differ between treatment groups (layer I, F_(3,16) = 0.915, p = 0.456; layer II, F_(3,16) = 0.738, p = 0.544; layers III-VI, F_(3,16) = 1.002, p = 0.418) (Fig. 3B-D). Likewise, none of the treatments exerted a significant effect on neuronal numbers in layers II (F_(3,16) = 0.084; p = 0.968) and III-VI (F_(3,16) = 0.203; p = 0.893) in this region (Fig. 3E,F).

Hippocampal formation

Volumetric analysis in the dentate gyrus revealed a significant effect of treatment on all layers (molecular layer, F_(3,16) = 4.073, p = 0.025; granule cell layer, F_(3,16) = 6.286, p = 0.005; hilus, F_(3,16) = 34.349, p < 0.001) (Fig. 4 A). The only detectable difference in the molecular layer was a reduction in the volumes of DEX-treated rats versus controls (p = 0.032); in the granule cell layer, the only significant volume reduction found was between the ADX and control (p = 0.005) and the ADX and CORT-treated (p = 0.03) groups. Hilus volume was reduced in all groups compared with controls (p < 0.001 in all cases); in addition, DEX-treated rats showed significantly different volumes compared with CORT-treated (p = 0.017) and ADX (p = 0.022) animals. The number of neurons in the granule cell layer of DEX-treated rats was significantly reduced compared with controls (20% lower, F_(3,16) = 13.083, p = 0.001); ADX animals showed significantly fewer neurons than controls (25% lower, p < 0.001) and CORT-treated rats (14% lower, p = 0.039) (Fig. 4 D).

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Figure 4.

Stereological data from the hippocampal formation. A-C, Mean volumes of the different layers of the dentate gyrus, CA3, and CA1 regions, respectively. D, Estimated number of neurons in the granule [dentate gyrus (DG)] and pyramidal (CA3 and CA1) layers. Note that, in A and D, the numerical values for the leftward bars (molecular layer and granule cell layer, respectively) should be read off against the axis on the left, whereas all other data should be read off against the right one. ^*p < 0.05 versus every other group; ^#p < 0.05 versus CON and versus ADX; ⁺⁺p < 0.05 versus CON; ^##p < 0.05 versus CON and versus CORT. Error bars represent SEM.

Treatment with DEX led to significant volume changes in the of strata of CA3 (stratum oriens, F_(3,16) = 15.789, p < 0.001; pyramidal cell layer, F_(3,16) = 6.031, p = 0.006; stratum radiatum, F_(3,16) = 8.342, p = 0.006) (Fig. 4B). Within the most superficial layer, DEX caused a significant volume reduction compared with other treatments (vehicle, p < 0.001; CORT, p = 0.003; ADX, p = 0.002); volumetric differences in the pyramidal cell layer and stratum radiatum were observed after DEX treatment with respect to controls (pyramidal cell layer, p = 0.010; stratum radiatum, p = 0.004) and ADX (pyramidal cell layer, p = 0.011; stratum radiatum, p = 0.003). In addition, the number of CA3 pyramidal neurons was reduced in DEX-treated rats (F_(3,16) = 5.633; p < 0.008) compared with control (p = 0.036) and ADX (p = 0.032) rats (Fig. 4D).

The volumes of the stratum oriens, pyramidal cell layer, and stratum radiatum of the CA1 did not differ between treatment groups (stratum oriens, F_(3,16) = 0.353, p = 0.787; pyramidal cell layer, F_(3,16) = 1.267, p = 0.319; stratum radiatum, F_(3,16) = 0.053, p = 0.983) (Fig. 4C). Likewise, none of the treatments exerted a significant effect on neuronal numbers within the CA1 subfield (F_(3,16) = 0.140; p = 0.934).

Precision of the estimates

The coefficients of error for volume and cell number estimates were all within the optimal range (<0.10), as detailed in supplemental Table 1 (available at www.jneurosci.org as supplemental material).

Discussion

Working memory, defined as the ability to transiently retain information that may be used to guide subsequent actions (Goldman-Rakic, 1995), is a characteristic function of the PFC. This concept, originally based on studies in humans and other primates (Greenlee et al., 2000; Petrides, 2000; Rowe and Passingham, 2001), has been shown to apply to rats using a variety of rigorous tests, including the radial-arm maze (Fritts et al., 1998), a matching-to-sample task on a T-maze (Dias and Aggleton, 2000), a delayed-response task (Mizoguchi et al., 2000), or the spatial working memory version of the Morris water maze (Kesner, 2000). The key feature of these tests is that the information presented is manipulated before the subject is tested for its ability to make an appropriate behavioral response; these tasks therefore contrast sharply with simple short-term memory tasks involving recognition and recall, abilities that are spared from impairment after PFC damage (Lacroix et al., 2002).

The spatial working memory version of the water maze was chosen to assess working memory performance in this study because it uses the same experimental set up and recruits a similar range of skills (swimming, visuospatial orientation) as does the Morris water maze, the classical test of hippocampus-dependent spatial reference memory. In the present study, we found that spatial working memory is (1) unaffected in corticosteroid-free (ADX) rats whose MR and GR remain unoccupied, (2) severely impaired in rats with selective and chronic activation of GR (through the administration of DEX), and (3) not affected by chronic treatment with the mixed MR/GR agonist CORT.

The likely insensitivity of the Morris water maze test of working memory to discriminate impairments in PFC-dependent working memory from deficits in PFC-independent short-term memory (Lacroix et al., 2002) may be overcome by applying tests of response flexibility. In such tests, subjects with PFC damage fail to perceive and adapt to new informational cues and persistently use behavioral strategies that are based on “old rules” that are no longer applicable (Salazar et al., 2004). A number of paradigms may be used to test behavioral adaptation, including the operant chamber (Salazar et al., 2004), T-maze (Dias and Aggleton, 2000), and Morris water maze (Lacroix et al., 2002). Using the last of these, we found that mPFC function is significantly disrupted in rats that had been ADX or exposed to chronic corticosteroid (CORT or DEX) treatments; of these three treatments, the GR agonist DEX caused the greatest impairment of PFC-dependent function.

Interesting insights into the role of corticosteroids in PFC-dependent functions can be gleaned by considering the combined results obtained in the spatial memory and reverse learning tasks. The finding that ADX and adrenal-intact control rats perform equally well and excel over DEX-treated subjects on the spatial working memory test could be interpreted to suggest that corticosteroids are not essential for mPFC function. This view is refuted by the observation that ADX animals show impaired performance on the reverse learning task; this demonstrates that corticosteroid receptor occupation is required for optimal behavioral flexibility. A similar conclusion was reached in another study, which showed the essential role of glucocorticoids in the delayed non-match-to-sample task, another test of mPFC-dependent function (Mizoguchi et al., 2004).

The PFC and hippocampus share several similarities and roles: (1) both participate in regulation of the HPA axis (Herman et al., 1996; Sullivan and Gratton, 2002), (2) both express MR and GR (Van Eekelen and de Kloet, 1992; Diorio et al., 1993; Kawata et al., 1998; Patel et al., 2000), and (3) both are involved in the processing of spatial information (Lee and Kesner, 2003). An additional interesting observation is that stress influences the induction of long-term potentiation (LTP) and long-term depression via a direct pathway between the PFC and hippocampus (Jay et al., 1995; Rocher et al., 2004). In contrast, the hippocampus displays higher MR and GR densities than the PFC (Kawata et al., 1998) and is more responsive to corticosteroids than the cortex (Kitchener et al., 2004). Here, it is relevant to mention that, although the precise distribution of MR and GR within the layers of the PFC of rodents is not known, available evidence suggests that (1) layers II/III of the PFC seems to be endowed with relatively high levels of both receptor [for MR, see Ahima et al. (1991) and van Eekelen et al. (1991); for GR, see Ahima and Harlan (1990), Cintra et al. (1994), and Morimoto et al. (1996)], and (2) there is an overall predominance of GR over MR in cortical structures (Ahima et al., 1990, 1991; Roland et al., 1995). We were thus prompted to compare the presently obtained data on the mPFC with those available for the hippocampus in which similar experimental paradigms were used. Notably, chronic DEX treatment, shown to impair PFC-dependent working memory and behavioral flexibility in this study, also impairs spatial reference memory, a function dependent of hippocampal integrity (our unpublished observations). Similarly, chronic CORT administration, which is known to interfere with hippocampal-dependent tasks (Coburn-Litvak et al., 2003), also reduces performance on tasks ascribed to the mPFC (present study). Last, ADX rats show deficits in tests of reference memory (Vaher et al., 1994; Conrad et al., 1997) and, as shown herein, display impaired behavioral flexibility despite an intact spatial working memory. By consolidating all of these findings, we now suggest that GR-activating corticosteroids (DEX or high CORT levels) are causally related to working memory deficits and that MR occupation (by basal CORT levels) is crucial for optimal mPFC function; this interpretation concords with current views on the dichotomous roles of MR and GR in the regulation of hippocampal structure and function (Sousa et al., 1999).

Our study also provides evidence for corticosteroid-induced changes in mPFC structure. With the aid of stereological tools, we present the first demonstration of DEX-induced neuronal loss in layer II of all mPFC regions; the fact that no other layer is affected indicates that the glucocorticoid acts in a highly specific manner. Importantly, DEX did not induce neuronal loss in the retrosplenial cortex; this area, which is endowed with corticosteroid receptors (Roland et al., 1995; Kawata et al., 1998; Patel et al., 2000), represents the posterior extension of the cingulate cortex and provides guidance information that is crucial for the performance of spatial reference and working memory tasks (Vann and Aggleton, 2004). It is also notable that structural parameters within the motor cortex, a nonlimbic region, are not affected by chronic DEX treatment.

In contrast to DEX (a GR-selective agonist), high levels of CORT (which saturates GR but also activates MR) (Reul and de Kloet, 1985; Spencer et al., 1990) did not cause a significant reduction in mPFC neuron numbers. This observation is similar to that made previously in the hippocampal dentate gyrus (Sousa et al., 1999) and reproduced herein and accords with other findings that the neurotoxic actions of GR ligands can be attenuated by concomitant activation of MR (Sousa et al., 1999; Almeida et al., 2000; Crochemore et al., 2005). Nevertheless, it should be noted that other area-specific factors are likely to play a role in determining the survival of specific cell types. As an example, although ADX leads to severe neuronal death in the hippocampus (Sloviter et al., 1989; Hu et al., 1997; Sousa et al., 1997), it does not lead to neuronal loss in the mPFC. From this, it follows that the mechanisms leading to ADX-induced impairments in PFC-dependent cognitive function are not likely to involve atrophy of PFC neurons; rather, impaired function may result from alterations to the afferent connections to the PFC, as discussed below.

After correcting for differential shrinkage, our results show that both DEX and CORT resulted in significant reductions in mPFC volumes, particularly in layer II. Although volumetric reductions in DEX-treated animals may be partially ascribed to decreased cell numbers, other mechanisms seem to be implicated in the CORT-induced changes observed. Our current hypothesis is that CORT leads to atrophy of the dendritic trees that abound in this area. This view is consonant with reports that chronic exposure to CORT (Wellman, 2001) and stress (Radley et al., 2004) leads to a retraction of the apical trees of PFC layer II/III pyramidal cells; in a more recent study, Radley et al. (2005) demonstrated that chronic stress induces a significant loss of spines in these neurons. Moreover, the selective hyperphosphorylation of ERK1/2 (extracellular signal-regulated protein kinases 1/2) in dendrites of the higher prefrontocortical layers after exposure of rats to chronic stress has been linked to stress-associated dendritic atrophy (Trentani et al., 2002).

The particular sensitivity of mPFC layer II to prolonged hypercortisolemia, as revealed by our stereological measurements, is striking. Because this layer represents the main receptive field of the mPFC, it would appear that deafferentation of this layer might trigger both the structural changes and the behavioral deficits seen after alterations in the corticosteroid milieu, an interpretation supported by the observation that synaptic contacts are essential for the shaping and reorganization of dendritic trees (Mizrahi and Libersat, 2002). As alluded to earlier, the vulnerability of mPFC layer II cells to glucocorticoids is likely to result from changes in other glucocorticoid target areas. In addition to the hippocampus, other limbic structures such as the mediodorsal nucleus of the thalamus, the ventral tegmental area (VTA), and the amygdala also project to this area; although nothing is known about how corticosteroids influence VTA and thalamic architecture, recent studies have shown that corticosteroid effects on PFC function involve activation of amygdaloid neurons (Roozendaal et al., 2004). Interestingly, recent studies have shown that corticosteroids/stress also induces structural changes in the dendrites of amygdalar neurons (Vyas et al., 2002) and that stress contemporaneously inhibits LTP in the basolateral amygdala-mPFC pathway (Maroun and Richter-Levin, 2003).

Together, these results call for a shift in the present focus on the effects of corticosteroid-induced plasticity in the hippocampus to a more systems-based approach in which interactions between corticosteroid-sensitive brain areas are analyzed. The present results provide a morphological basis for how corticosteroid status (and presumably stress) can impact working memory; they contribute to our increased understanding of the neurobiological bases of a number of disease states in which PFC dysfunction is implicated (Lewis et al., 2004; Monchi et al., 2004; Shad et al., 2004); in particular, these findings may be relevant to depression (cf. Rogers et al., 2004). Indeed, the presently described corticosteroid-induced damage to the mPFC fits with previous postmortem data from subjects with major depression and bipolar disorder (Rajkowska, 2000). That work revealed specific histopathological changes (including cell atrophy and the loss of glia but not neurons) in the dorsolateral PFC, the human homolog of the rodent mPFC.

Footnotes

References