Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE).

Author information

Affiliations

  • 1. Departamento de Bioquímica y Biología Molecular, Universitat de Valencia, Spain.
      Authors
    • Martínez-Pastor MT 1
    (1 author)

ORCIDs linked to this article

The EMBO Journal, 01 May 1996, 15(9):2227-2235
https://doi.org/10.1002/j.1460-2075.1996.tb00576.x PMID: 8641288 PMCID: PMC450147

Free full text in Europe PMC

Abstract 

The MSN2 and MSN4 genes encode homologous and functionally redundant Cys2His2 zinc finger proteins. A disruption of both MSN2 and MSN4 genes results in a higher sensitivity to different stresses, including carbon source starvation, heat shock and severe osmotic and oxidative stresses. We show that MSN2 and MSN4 are required for activation of several yeast genes such as CTT1, DDR2 and HSP12, whose induction is mediated through stress-response elements (STREs). Msn2p and Msn4p are important factors for the stress-induced activation of STRE dependent promoters and bind specifically to STRE-containing oligonucleotides. Our results suggest that MSN2 and MSN4 encode a DNA-binding component of the stress responsive system and it is likely that they act as positive transcription factors.

Free full text 

Logo of embojLink to Publisher's site
EMBO J. 1996 May 1; 15(9): 2227–2235.
PMCID: PMC450147
PMID: 8641288

The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE).

M T Martínez-Pastor, G Marchler, C Schüller, A Marchler-Bauer, H Ruis, and F Estruch
Author information Copyright and License information Disclaimer

Abstract

The MSN2 and MSN4 genes encode homologous and functionally redundant Cys2His2 zinc finger proteins. A disruption of both MSN2 and MSN4 genes results in a higher sensitivity to different stresses, including carbon source starvation, heat shock and severe osmotic and oxidative stresses. We show that MSN2 and MSN4 are required for activation of several yeast genes such as CTT1, DDR2 and HSP12, whose induction is mediated through stress-response elements (STREs). Msn2p and Msn4p are important factors for the stress-induced activation of STRE dependent promoters and bind specifically to STRE-containing oligonucleotides. Our results suggest that MSN2 and MSN4 encode a DNA-binding component of the stress responsive system and it is likely that they act as positive transcription factors.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
 
icon of scanned page 2227
2227
icon of scanned page 2228
2228
icon of scanned page 2229
2229
icon of scanned page 2230
2230
icon of scanned page 2231
2231
icon of scanned page 2232
2232
icon of scanned page 2233
2233
icon of scanned page 2234
2234
icon of scanned page 2235
2235
 

Images in this article

Image
Image
on p.2229
Image
Image
on p.2230
Image
Image
on p.2231
Image
Image
on p.2232
Image
Image
on p.2232
Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ammerer G. Sex, stress and integrity: the importance of MAP kinases in yeast. Curr Opin Genet Dev. 1994 Feb;4(1):90–95. [Abstract] [Google Scholar]
  • Bienz M, Pelham HR. Mechanisms of heat-shock gene activation in higher eukaryotes. Adv Genet. 1987;24:31–72. [Abstract] [Google Scholar]
  • Boorstein WR, Craig EA. Regulation of a yeast HSP70 gene by a cAMP responsive transcriptional control element. EMBO J. 1990 Aug;9(8):2543–2553. [Europe PMC free article] [Abstract] [Google Scholar]
  • Brewster JL, de Valoir T, Dwyer ND, Winter E, Gustin MC. An osmosensing signal transduction pathway in yeast. Science. 1993 Mar 19;259(5102):1760–1763. [Abstract] [Google Scholar]
  • Choo Y, Klug A. Selection of DNA binding sites for zinc fingers using rationally randomized DNA reveals coded interactions. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11168–11172. [Europe PMC free article] [Abstract] [Google Scholar]
  • Choo Y, Sánchez-García I, Klug A. In vivo repression by a site-specific DNA-binding protein designed against an oncogenic sequence. Nature. 1994 Dec 15;372(6507):642–645. [Abstract] [Google Scholar]
  • Estruch F, Carlson M. Increased dosage of the MSN1 gene restores invertase expression in yeast mutants defective in the SNF1 protein kinase. Nucleic Acids Res. 1990 Dec 11;18(23):6959–6964. [Europe PMC free article] [Abstract] [Google Scholar]
  • Estruch F, Carlson M. Two homologous zinc finger genes identified by multicopy suppression in a SNF1 protein kinase mutant of Saccharomyces cerevisiae. Mol Cell Biol. 1993 Jul;13(7):3872–3881. [Europe PMC free article] [Abstract] [Google Scholar]
  • Gallwitz D, Sures I. Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1980 May;77(5):2546–2550. [Europe PMC free article] [Abstract] [Google Scholar]
  • Han J, Lee JD, Bibbs L, Ulevitch RJ. A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells. Science. 1994 Aug 5;265(5173):808–811. [Abstract] [Google Scholar]
  • Herskowitz I. MAP kinase pathways in yeast: for mating and more. Cell. 1995 Jan 27;80(2):187–197. [Abstract] [Google Scholar]
  • Kamada Y, Jung US, Piotrowski J, Levin DE. The protein kinase C-activated MAP kinase pathway of Saccharomyces cerevisiae mediates a novel aspect of the heat shock response. Genes Dev. 1995 Jul 1;9(13):1559–1571. [Abstract] [Google Scholar]
  • Kobayashi N, McEntee K. Evidence for a heat shock transcription factor-independent mechanism for heat shock induction of transcription in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1990 Sep;87(17):6550–6554. [Europe PMC free article] [Abstract] [Google Scholar]
  • Kobayashi N, McEntee K. Identification of cis and trans components of a novel heat shock stress regulatory pathway in Saccharomyces cerevisiae. Mol Cell Biol. 1993 Jan;13(1):248–256. [Europe PMC free article] [Abstract] [Google Scholar]
  • Kuge S, Jones N. YAP1 dependent activation of TRX2 is essential for the response of Saccharomyces cerevisiae to oxidative stress by hydroperoxides. EMBO J. 1994 Feb 1;13(3):655–664. [Europe PMC free article] [Abstract] [Google Scholar]
  • Kyriakis JM, Banerjee P, Nikolakaki E, Dai T, Rubie EA, Ahmad MF, Avruch J, Woodgett JR. The stress-activated protein kinase subfamily of c-Jun kinases. Nature. 1994 May 12;369(6476):156–160. [Abstract] [Google Scholar]
  • Lindquist S, Craig EA. The heat-shock proteins. Annu Rev Genet. 1988;22:631–677. [Abstract] [Google Scholar]
  • Mager WH, Ferreira PM. Stress response of yeast. Biochem J. 1993 Feb 15;290(Pt 1):1–13. [Europe PMC free article] [Abstract] [Google Scholar]
  • Mager WH, De Kruijff AJ. Stress-induced transcriptional activation. Microbiol Rev. 1995 Sep;59(3):506–531. [Europe PMC free article] [Abstract] [Google Scholar]
  • Marchler G, Schüller C, Adam G, Ruis H. A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions. EMBO J. 1993 May;12(5):1997–2003. [Europe PMC free article] [Abstract] [Google Scholar]
  • McClanahan T, McEntee K. DNA damage and heat shock dually regulate genes in Saccharomyces cerevisiae. Mol Cell Biol. 1986 Jan;6(1):90–96. [Europe PMC free article] [Abstract] [Google Scholar]
  • Morimoto RI, Sarge KD, Abravaya K. Transcriptional regulation of heat shock genes. A paradigm for inducible genomic responses. J Biol Chem. 1992 Nov 5;267(31):21987–21990. [Abstract] [Google Scholar]
  • Pavletich NP, Pabo CO. Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A. Science. 1991 May 10;252(5007):809–817. [Abstract] [Google Scholar]
  • Piper PW. Molecular events associated with acquisition of heat tolerance by the yeast Saccharomyces cerevisiae. FEMS Microbiol Rev. 1993 Aug;11(4):339–355. [Abstract] [Google Scholar]
  • Praekelt UM, Meacock PA. HSP12, a new small heat shock gene of Saccharomyces cerevisiae: analysis of structure, regulation and function. Mol Gen Genet. 1990 Aug;223(1):97–106. [Abstract] [Google Scholar]
  • Rose M, Botstein D. Construction and use of gene fusions to lacZ (beta-galactosidase) that are expressed in yeast. Methods Enzymol. 1983;101:167–180. [Abstract] [Google Scholar]
  • Rothstein RJ. One-step gene disruption in yeast. Methods Enzymol. 1983;101:202–211. [Abstract] [Google Scholar]
  • Rouse J, Cohen P, Trigon S, Morange M, Alonso-Llamazares A, Zamanillo D, Hunt T, Nebreda AR. A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins. Cell. 1994 Sep 23;78(6):1027–1037. [Abstract] [Google Scholar]
  • Ruis H, Schüller C. Stress signaling in yeast. Bioessays. 1995 Nov;17(11):959–965. [Abstract] [Google Scholar]
  • Schena M, Picard D, Yamamoto KR. Vectors for constitutive and inducible gene expression in yeast. Methods Enzymol. 1991;194:389–398. [Abstract] [Google Scholar]
  • Schüller C, Brewster JL, Alexander MR, Gustin MC, Ruis H. The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces cerevisiae CTT1 gene. EMBO J. 1994 Sep 15;13(18):4382–4389. [Europe PMC free article] [Abstract] [Google Scholar]
  • Smith DB, Johnson KS. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 1988 Jul 15;67(1):31–40. [Abstract] [Google Scholar]
  • Spevak W, Fessl F, Rytka J, Traczyk A, Skoneczny M, Ruis H. Isolation of the catalase T structural gene of Saccharomyces cerevisiae by functional complementation. Mol Cell Biol. 1983 Sep;3(9):1545–1551. [Europe PMC free article] [Abstract] [Google Scholar]
  • Susek RE, Lindquist S. Transcriptional derepression of the Saccharomyces cerevisiae HSP26 gene during heat shock. Mol Cell Biol. 1990 Dec;10(12):6362–6373. [Europe PMC free article] [Abstract] [Google Scholar]
  • Tamai KT, Liu X, Silar P, Sosinowski T, Thiele DJ. Heat shock transcription factor activates yeast metallothionein gene expression in response to heat and glucose starvation via distinct signalling pathways. Mol Cell Biol. 1994 Dec;14(12):8155–8165. [Europe PMC free article] [Abstract] [Google Scholar]
  • Thiele DJ. Metal-regulated transcription in eukaryotes. Nucleic Acids Res. 1992 Mar 25;20(6):1183–1191. [Europe PMC free article] [Abstract] [Google Scholar]
  • Thomas BJ, Rothstein R. Elevated recombination rates in transcriptionally active DNA. Cell. 1989 Feb 24;56(4):619–630. [Abstract] [Google Scholar]
  • Varela JC, Praekelt UM, Meacock PA, Planta RJ, Mager WH. The Saccharomyces cerevisiae HSP12 gene is activated by the high-osmolarity glycerol pathway and negatively regulated by protein kinase A. Mol Cell Biol. 1995 Nov;15(11):6232–6245. [Europe PMC free article] [Abstract] [Google Scholar]
  • Werner-Washburne M, Stone DE, Craig EA. Complex interactions among members of an essential subfamily of hsp70 genes in Saccharomyces cerevisiae. Mol Cell Biol. 1987 Jul;7(7):2568–2577. [Europe PMC free article] [Abstract] [Google Scholar]
  • Wieser R, Adam G, Wagner A, Schüller C, Marchler G, Ruis H, Krawiec Z, Bilinski T. Heat shock factor-independent heat control of transcription of the CTT1 gene encoding the cytosolic catalase T of Saccharomyces cerevisiae. J Biol Chem. 1991 Jul 5;266(19):12406–12411. [Abstract] [Google Scholar]
  • Wu AL, Moye-Rowley WS. GSH1, which encodes gamma-glutamylcysteine synthetase, is a target gene for yAP-1 transcriptional regulation. Mol Cell Biol. 1994 Sep;14(9):5832–5839. [Europe PMC free article] [Abstract] [Google Scholar]
Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

Full text links 

Read article at publisher's site: https://doi.org/10.1002/j.1460-2075.1996.tb00576.x

Read article for free, from open access legal sources, via Unpaywall: https://europepmc.org/articles/pmc450147?pdf=renderUnpaywall

Citations & impact 

Impact metrics

625
Citations
Jump to Citations
5
Data citations
Jump to Data

Citations of article over time

Smart citations by scite.ai
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by EuropePMC if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1002/j.1460-2075.1996.tb00576.x

Supporting
Mentioning
Contrasting
35
973
5

Article citations

Go to all (625) article citations

Data 

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.